Effects of osteoblastic growth peptide on the proliferation and total protein content in cultured human periodontal ligament cells

XU Ling; et al

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Effects of osteoblastic growth peptide on the proliferation and total protein content in cultured human periodontal ligament cells

更新时间：2024-01-17

- Effects of osteoblastic growth peptide on the proliferation and total protein content in cultured human periodontal ligament cells
- Journal of Chongqing Medical University Vol. 34, Issue 4, Pages: 420-423(2009)
- 作者机构：
  
  1. 重庆医科大学附属口腔医院修复科
  2. 重庆医科大学附属口腔医院牙周科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R78
- Published：2009，
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[1]徐凌,黄姣,向学熔.成骨生长肽对人牙周膜细胞增殖和总蛋白含量的影响[J].重庆医科大学学报,2009,34(04):420-423.

XU Ling, et al. Effects of osteoblastic growth peptide on the proliferation and total protein content in cultured human periodontal ligament cells. [J]. Journal of Chongqing Medical University 34(4):420-423(2009)
[1]徐凌,黄姣,向学熔.成骨生长肽对人牙周膜细胞增殖和总蛋白含量的影响[J].重庆医科大学学报,2009,34(04):420-423. DOI：

XU Ling, et al. Effects of osteoblastic growth peptide on the proliferation and total protein content in cultured human periodontal ligament cells. [J]. Journal of Chongqing Medical University 34(4):420-423(2009) DOI：

摘要

目的:研究成骨生长肽（Osteogenic growth peptide

OGP）对体外培养的人牙周膜细胞增殖和细胞总蛋白含量的影响。方法:在体外培养的人牙周膜细胞中加入不同浓度的OGP（10-11～10-7mol/L）

用MTT法检测细胞的增殖率;考马斯亮蓝染色法检测细胞内总蛋白含量的变化。结果:OGP在该浓度范围内时

对人牙周膜细胞的增殖率有明显的促进作用（P<0.05）

并且在该浓度范围内时

能明显增加人牙周膜细胞总蛋白的合成（P<0.05）

最佳作用浓度为10-9mol/L。结论:OGP可促进人牙周膜细胞的增殖活性

同时可以增强细胞总蛋白的合成

提示OGP在牙周组织再生领域中具有良好的应用前景。

Abstract

Objective:To elucidate the effect of osteogenic growth peptide(OGP) on proliferation and protein synthesis of human PDLCs in vitro.Methods:Human PDLCs were cultured individually in medium contained various concentrations of OGP;and cell proliferation quantity and protein synthesis were examined by MTT and coom asie brilliant blue staining.Results:In vitro

the addition of OGP in cultures resulted in an increase of cell proliferation(P<0.05)and protein synthesi(sP<0.05).All OGP concentrations used(10-11~10-7 mol/L) were effective but the maximum response was obtained with 10-9mol/L OGP.Conclusion:OGP stimulates proliferation and protein synthesis of human PDLCs in vitro

suggesting that OGP is a promising growth factor in the region of periodontal tissue regeneration.

关键词

成骨生长肽人牙周膜细胞细胞增殖总蛋白含量

Keywords

Osteogenic growth peptidehuman periodontal ligament cellsproliferationTotal protein contents

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Related Institution

Department of Periodontics,the Affiliated Stomatologic Hospital,Chongqing Medical University

Prosthodontics,Department of the Affiliated Hospital of Stomatological,Chongqing Medical University

Department of Hepatobiliary Surgery,the Second Affiliated Hospital,Chongqing Medical University

重庆市肿瘤医院肿瘤研究所

重庆医科大学检验系

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