Simultaneous determination of nine types of conjugated bile acids in bile by high performance liquid chromatography

WANG Wen-juan; et al.

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Simultaneous determination of nine types of conjugated bile acids in bile by high performance liquid chromatography

更新时间：2024-01-17

- Simultaneous determination of nine types of conjugated bile acids in bile by high performance liquid chromatography
- Journal of Chongqing Medical University Vol. 34, Issue 1, Pages: 59-62(2009)
- 作者机构：
  
  1. 重庆医科大学检验学临床检验诊断学教育部重点实验室
  2. 第三军医大学西南医院肝胆外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R446.1
- Published：2009，
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[1]王文娟,邓文平,王猛,陈耿,丁敏.高效液相色谱法同时测定胆汁中9种结合型胆汁酸[J].重庆医科大学学报,2009,34(01):59-62.

WANG Wen-juan, et al.. Simultaneous determination of nine types of conjugated bile acids in bile by high performance liquid chromatography. [J]. Journal of Chongqing Medical University 34(1):59-62(2009)
[1]王文娟,邓文平,王猛,陈耿,丁敏.高效液相色谱法同时测定胆汁中9种结合型胆汁酸[J].重庆医科大学学报,2009,34(01):59-62. DOI：

WANG Wen-juan, et al.. Simultaneous determination of nine types of conjugated bile acids in bile by high performance liquid chromatography. [J]. Journal of Chongqing Medical University 34(1):59-62(2009) DOI：

摘要

目的:建立测定人胆汁中结合型胆汁酸的HPLC-紫外检测法。方法:胆汁样品经固相萃取

采用WatersNovaC18色谱柱

柱温为25℃

流动相为甲醇∶磷酸盐缓冲液（60mmol/L

pH3.3）（66∶34

v/v）

流速0.8ml/min

检测波长197nm。结果:9种结合型胆汁酸在20min内分离完全

各胆汁酸在浓度为8

000μmol/L范围内线性良好

r为0.9991

.9999;最低检测限为1.25

.13μmol/L;该方法日内变异在3.77%

.20%之间

日间变异在6.73%

7.11%之间

各胆汁酸的回收率在88.23%

09.75%之间。结论:本方法准确、灵敏、快速

适用于胆汁中结合型胆汁酸水平的实验室研究和临床常规检测。

Abstract

Objective:To develop a HPLC method with ultraviolet detection to determine nine types of conjugated bile acids in human bile.Methods:Bile acids in bile juice were extracted using Sep-Pak C18 cartridges.The analytes were eluted using methanol/phosphate buffer(60 μmol/L

pH3.3)(66:34

v/v)as mobile phase on a Waters Nova C18 column.The flow rate was 0.8 ml/min.The column was kept at 25℃.The eluate was monitored by a ultraviolet detection at 197nm.Results:The nine types of conjugated bile acids were completely analyzed in twenty minitus by this method.The assay was linear in the range 8~100 0 μmol/L

with the correlation coeffients for linear regression from 0.9991 to 0.999 9.The detection limits ranged from 1.25 to 3.12 μmol/L.The within-day precision was below 8.20%

and the between-day precision was below 17.11%.The recoveries were 88.23%~109.75%.Conclusion:This method is accurate

sensitive

rapid and suitable for the rountine clinical practice and research practice.

关键词

胆汁胆汁酸色谱法

Keywords

BileBile acidsChromatography

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Key Laboratory of Laboratory Medical Diagnostics,Ministry of Education,Chongqing Medical University

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