Protective effect of extract from rhubarb and alismae rhizome on acute liver injury induced by diethylene glycol

ZHU Shen-yin; et al

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Protective effect of extract from rhubarb and alismae rhizome on acute liver injury induced by diethylene glycol

更新时间：2024-01-17

- Protective effect of extract from rhubarb and alismae rhizome on acute liver injury induced by diethylene glycol
- Journal of Chongqing Medical University Vol. 34, Issue 2, Pages: 212-215(2009)
- 作者机构：
  
  1. 重庆医科大学附属第一医院临床药理研究室
  2. 中国协和医科大学&中国医学科学院药物研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5
- Published：2009，
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[1]朱深银,周远大,杜冠华.大黄和泽泻提取物对二甘醇致小鼠肝脏损伤的保护作用[J].重庆医科大学学报,2009,34(02):212-215.

ZHU Shen-yin, et al. Protective effect of extract from rhubarb and alismae rhizome on acute liver injury induced by diethylene glycol. [J]. Journal of Chongqing Medical University 34(2):212-215(2009)
[1]朱深银,周远大,杜冠华.大黄和泽泻提取物对二甘醇致小鼠肝脏损伤的保护作用[J].重庆医科大学学报,2009,34(02):212-215. DOI：

ZHU Shen-yin, et al. Protective effect of extract from rhubarb and alismae rhizome on acute liver injury induced by diethylene glycol. [J]. Journal of Chongqing Medical University 34(2):212-215(2009) DOI：

摘要

目的:考察大黄和泽泻提取物对二甘醇（Diethylene glycol

DEG）致肝脏损伤的保护作用

并探讨其作用机制。方法:采用DEG（18.7ml/kg

ig）造成小鼠肝脏损伤模型。大黄醇提物0.8及0.4g/kg、大黄水提物0.8及0.4g/kg和泽泻醇提物3.6及1.8g/kg连续灌胃给药4d

第5dDEG染毒造模后1h

再分别灌胃给药1次

染毒24h后观察小鼠中毒表现;测定血清中丙氨酸基转移酶（Alanine aminotransferase

ALT）、天冬氨酸氨基转移酶（Aspartate aminotransferase

AST）、总胆红素（Total billirubin

TBIL）水平;取肝脏制备匀浆

测定超氧化物歧化酶（Superoxi dedismutase

SOD）、谷胱甘肽过氧化酶活性（Glutathion peroxidase

GSH-PX）、丙二醛含量（Malondialdehyde

MDA）变化。结果:小鼠二甘醇染毒后

出现明显中毒表现

肝脏体重比增加

血清中ALT、AST和TBIL水平显著升高

肝组织SOD和GSH-PX活性降低、MDA含量增加;大黄醇提物、大黄水提物和泽泻醇提取物给药组小鼠中毒表现明显减轻

肝脏体重比减少

血清中ALT、AST和TBIL水平明显降低

肝组织SOD和GSH-PX活性升高、MDA含量降低。结论:大黄和泽泻提取物对DEG所致的小鼠急性肝脏损伤具有明显的保护作用

该作用与其改善肝脏抗氧化酶活性及抑制脂质过氧化反应有关。

Abstract

Objective:To explore protective effect of extract from rhubarb and alismae rhizome on acute liver injury induced by diethylene glycol(DEG)and its mechanism.Methods:Animal model of acute liver injury induced by DEG(18.7 ml/kg)was applied to investigate the protective effect of extract from rhubarb and alismae rhizome.Mice were administered intragastrically with n-butanol extract from rhubarb 0.8 and 0.4 g/kg

aqueous extract from rhubarb 0.8 and 0.4 g/kg and ethanol extract from alismae rhizome 3.6 and 1.8 g/kg for 4 days

respectively.DEG(18.7 ml/kg)was administered intragastrically to the mice 1 hour before extract from rhubarb and alismae rhizome administration on day 5.The manifestation of the poisoned mice were observed

the serum was prepared and the mice were then sacrificed after ig DEG for 24 h.The contents of ALT

AST and TBIL in serum of mice were assayed

and the liver tissues were collected and the ho-mogenate was prepared

the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)and the contents of malon-aldehyde(MDA)in liver homogenate were also detected.Results:After the administration of DEG

the poisoned manifestations of the mice were obviously visible

the ratio of liver to body weight increased

the contents of ALT

AST and TBIL in serum significantly in-creased

the activities of SOD and GSH-PX in liver homogenate decreased

and the contents of MDA in liver homogenate increased.Com-pared with that in DEG model group

in extract from rhubarb and alismae rhizome treatment groups

the poisoned manifestations were ob-viously relieved

the ratio of liver to body weight decreased

the contents of ALT and AST and TBIL significantly lowered

the activities of SOD and GSH-PX were also markedly improved and the contents of MDA significantly decreased.Conclusion:DEG can obviously dam-age liver

but extracts from rhubarb and alismae rhizome have an obvious protective effect against DEG-induced acute liver injury

and the protection role is related to the improvement of antioxidation enzyme activities and suppression of lipid peroxidation.

关键词

二甘醇肝脏损伤大黄泽泻脂质过氧化

Keywords

Diethylene glycolLiver injuryRhubarbAlismae rhizomelipid peroxidation

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