Effects of FuYuan capsule on the expression of TIMP-1 and TGF-β1 in liver of hepatic fibrosis in Rats

XIONG Sheng-chi; et al

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Effects of FuYuan capsule on the expression of TIMP-1 and TGF-β1 in liver of hepatic fibrosis in Rats

更新时间：2024-01-17

- Effects of FuYuan capsule on the expression of TIMP-1 and TGF-β1 in liver of hepatic fibrosis in Rats
- Journal of Chongqing Medical University Vol. 34, Issue 2, Pages: 216-220(2009)
- 作者机构：
  
  1. 重庆医科大学附属第一医院中西医结合科
  2. 重庆医科大学中医药学院中医临床教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5
- Published：2009，
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[1]熊盛池,李荣亨,王淑美,张素养.复元胶囊对肝纤维化大鼠肝脏中的TIMP-1和TGF-β1表达的影响[J].重庆医科大学学报,2009,34(02):216-220.

XIONG Sheng-chi, et al. Effects of FuYuan capsule on the expression of TIMP-1 and TGF-β1 in liver of hepatic fibrosis in Rats. [J]. Journal of Chongqing Medical University 34(2):216-220(2009)
[1]熊盛池,李荣亨,王淑美,张素养.复元胶囊对肝纤维化大鼠肝脏中的TIMP-1和TGF-β1表达的影响[J].重庆医科大学学报,2009,34(02):216-220. DOI：

XIONG Sheng-chi, et al. Effects of FuYuan capsule on the expression of TIMP-1 and TGF-β1 in liver of hepatic fibrosis in Rats. [J]. Journal of Chongqing Medical University 34(2):216-220(2009) DOI：

摘要

目的:研究复元胶囊对猪血清所致大鼠肝纤维化的保护作用及其机制。方法:将大鼠随机分为正常组

模型组

复元胶囊低、中、高剂量组

阳性对照组（复方鳖甲软肝片水溶液组）6组

每组8只。除正常组外

其余各组用猪血清

0.5ml/只

腹腔注射

每周两次（星期一

星期四）共10周

复制大鼠免疫性肝纤维化模型

以此同时给予复元胶囊低、中、高剂量组

阳性对照组相应药物干预。造模、给药结束后观察各组动物的肝组织病理学变化

并检测丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）的活性

检测血清透明质酸（HA）、Ⅲ型前胶原（PCⅢ）、Ⅳ型胶原（Ⅳ-C）及层粘连蛋白（LN）的含量。免疫组化显示基质金属蛋白酶组织抑制因子1（Tissue inhibitor of metalloproteinases-1

TIMP-1）及转化生长因子β1（Transforming growth facto rβ-1

TGF-β1）。结果:复元胶囊组血清中ALT、AST、HA、LN、IV-C、PCⅢ比模型组低

特别是复元胶囊高剂量组

差异有统计学意义（P<0.05）。模型组TIMP-1及TGF-β1的表达明显高于复元胶囊组

差异有统计学意义（P<0.05）。结论:复元胶囊对猪血清诱导的大鼠肝纤维化有明显保护作用。

Abstract

Objective:To study the effects of FuYuan capsule on rat liver fibrosis induced by pig serum.Methods:The rats were randomly divided into six groups

including the normal group

the model group

the low

middle and high dose FuYuan capsule group

and the positive control group(compound Biejiaruangan-chip solution).The rat models of immune liver fibrosis were induced with pig serum.Alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum were detected with autoumatic biochemical analyzer.Hyaluronan(HA)

laminin(LN).IV collagen(IV-C)and the typeⅢcollagen(PCⅢ)in serum were quantified by radiative-immune technology.The expression of TIMP-1(tissue inhibitor of metalloproteinases-1)and TGF-β1(transforming growth factorβ-1)in the liver were observed by immunohistochemical assay.Results:ALT

AST

IV-C and PCⅢin the model group were significantly higher than those in the other group(sP<0.05)

and there were significant difference between the model group and the FuYuancapsulegroup(sP<0.05)

especiallythehigh-dosegroup.Compared with that in model group

the expression of TIMP-1andTGF-β1 in the Fuyuan groups were significantly lowe(rP<0.05)

especially in the high-dose group.Conclusion:The protective effects of FuYuan capsule on the pig serum-induced liver fibrosis are remarkable.

关键词

复元胶囊肝纤维化基质金属蛋白酶组织抑制因子1转化生长因子β1

Keywords

Fuyuan capsuleliver fibrosisTissue inhibitor of metalloproteinases-1Transforming growth factorβ1

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