Simultaneous determination of berberine hydrochloride and baicalin in lanqin oral liquid by HPLC

HU Mao-hua; et al

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Simultaneous determination of berberine hydrochloride and baicalin in lanqin oral liquid by HPLC

更新时间：2024-01-17

- Simultaneous determination of berberine hydrochloride and baicalin in lanqin oral liquid by HPLC
- Journal of Chongqing Medical University Vol. 34, Issue 2, Pages: 210-211(2009)
- 作者机构：
  
  重庆医科大学药学院药物分析教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R286.0
- Published：2009，
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[1]胡茂华,钟玲,黄小玉,尚京川.HPLC法同时测定蓝芩口服液中盐酸小檗碱与黄芩苷的含量[J].重庆医科大学学报,2009,34(02):210-211.

HU Mao-hua, et al. Simultaneous determination of berberine hydrochloride and baicalin in lanqin oral liquid by HPLC. [J]. Journal of Chongqing Medical University 34(2):210-211(2009)
[1]胡茂华,钟玲,黄小玉,尚京川.HPLC法同时测定蓝芩口服液中盐酸小檗碱与黄芩苷的含量[J].重庆医科大学学报,2009,34(02):210-211. DOI：

HU Mao-hua, et al. Simultaneous determination of berberine hydrochloride and baicalin in lanqin oral liquid by HPLC. [J]. Journal of Chongqing Medical University 34(2):210-211(2009) DOI：

摘要

目的:建立同时测定蓝芩口服液中盐酸小檗碱和黄芩苷的含量方法。方法:采用高效液相色谱法

色谱柱:Zorbax XDB-C18柱（4.6×150mm

5μm）

流动相:甲醇-0.5%磷酸溶液梯度洗脱

比例为0～16.5min

甲醇38%～40%

流速1ml/min;16.8～18min

甲醇99%

流速1.6ml/min;18.1～22min

甲醇38%

流速1.6ml/min;22.1～23min

甲醇38%

流速1ml/min。检测波长为275nm

柱温30℃。结果:盐酸小檗碱在6.8～65.28μg/ml

黄芩苷在31.2～299.52μg/ml范围内与峰面积呈良好的线性关系。加样回收率在97.0%～101.3%

RSD小于2.0%。结论:本法操作简便、结果准确、分离效果好

可用于蓝芩口服液中盐酸小檗碱和黄芩苷含量的测定。

Abstract

Objective:To simultaneously determine the contents of berberine hydroehloride and baicalin in Lanqin Oral Liquid via an HPLC method.Method:The HPLC with a Cl8 column(4.6×150 mm

5μm)was used.The mobile phases were as follows:the methanol-0.5%orthophosphoric acid(0～16.5 min

methanol 38%～40%

V=1 ml/min;16.8～18 min

methanol 99%

V=1.6ml/min;18.1～22min

methanol 38%

V=1.6ml/min;22.1～23 min

methanol 38%

V=1 ml/min.).The wavelength for detection was located at 275nm.Result:The linear ranges of berberine hydrochloride and baicallin were 6.8～65.28μg/ml and 31.2～299.52μg/ml

respectively

both showing a good linar relationship

and the average spotting recoveries were higher than 97.0%.The coefficient of variation was smaller than 2.0%.Con-clusion:The method is convenient and can be used for controlling the quality of Lanqin Oral Liquid.

关键词

高效液相色谱法蓝芩口服液盐酸小檗碱黄芩苷

Keywords

HPLCLanqin oral liquidBerberine hydrochloridebaicalin

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