Expression analysis of microRNA on acute cerebral ischemia in rats

WEN Quan-qing; et al

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Expression analysis of microRNA on acute cerebral ischemia in rats

更新时间：2024-01-17

- Expression analysis of microRNA on acute cerebral ischemia in rats
- Journal of Chongqing Medical University Issue S1, Pages: 23-26(2008)
- 作者机构：
  
  郑州大学第一附属医院神经科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R743.3
- Published：2008，
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[1]文全庆,贾延劼,王明闯,赵二义,王留东,张博爱,刘洪波.大鼠脑缺血急性期脑组织miRNA的表达变化[J].重庆医科大学学报,2008(S1):23-26+40.

WEN Quan-qing, et al. Expression analysis of microRNA on acute cerebral ischemia in rats. [J]. Journal of Chongqing Medical University (S1):23-26(2008)
[1]文全庆,贾延劼,王明闯,赵二义,王留东,张博爱,刘洪波.大鼠脑缺血急性期脑组织miRNA的表达变化[J].重庆医科大学学报,2008(S1):23-26+40. DOI：

WEN Quan-qing, et al. Expression analysis of microRNA on acute cerebral ischemia in rats. [J]. Journal of Chongqing Medical University (S1):23-26(2008) DOI：

摘要

目的:观察脑缺血急性期大鼠脑组织小RNA（microRNA

miRNA）差异表达。方法:采用双颈总动脉结扎方法制备大鼠脑缺血模型（2VO）

脑缺血后30min

将动物断头取皮层脑组织

miRNA表达谱芯片检测miRNA差异表达。结果:与正常组比较

2VO术后60min大鼠皮层脑组织中35个已知miRNA表达上调2倍以上;89个表达下调2倍以上等

其中促进凋亡和影响细胞周期的miRNA

如miR-23、-24、-26、-30、-103、-107等显著上调

与神经干或前体细胞分化有关的let-7、miR-124、-128、-9等也显著上调。结论:miRNA在脑缺血早期表达即有明显差异

可能在大鼠脑缺血早期病理生理变化中发挥作用。

Abstract

Objective:To observe the change of microRNA(miRNA) expression on acute cerebral ischemia in rats. Methods:We adopted the permanent occlusion of bilateral common carotid arteries in Wistar rats to produce cerebral ischemia model (2VO).Then we examined miRNA expression of cortex tissue after 60 minutes of occlusion by miRNA chip. Results:Compared with normal rats

35 pieces of miRNA were up-regulated more than twice in the 2VO rats.And 89 pieces were down-regulated more than twice. Conclusion:The miRNA changed on acute cerebral ischemia in rats. It may play an important role in pathophysiology on acute cerebral ischemia.

关键词

小RNA脑缺血大鼠表达

Keywords

microRNAcerebral ischemiaratsexpression

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