Research on Nipah virus infection in equine of Yili, Xinjiang Uygur Autonomous Region

LIU Yan-xi; et al

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Research on Nipah virus infection in equine of Yili, Xinjiang Uygur Autonomous Region

更新时间：2024-01-17

- Research on Nipah virus infection in equine of Yili, Xinjiang Uygur Autonomous Region
- Journal of Chongqing Medical University Issue S1, Pages: 27-29(2008)
- 作者机构：
  
  重庆医科大学附属第一医院神经内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R512.99
- Published：2008，
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[1]刘妍汐,谢鹏,曾志磊,翟红,徐鸣明,张英英,余建萍,陈晓,彭丹,朱丹.新疆伊犁地区马的尼帕病毒感染情况研究[J].重庆医科大学学报,2008(S1):27-29.

LIU Yan-xi, et al. Research on Nipah virus infection in equine of Yili, Xinjiang Uygur Autonomous Region. [J]. Journal of Chongqing Medical University (S1):27-29(2008)
[1]刘妍汐,谢鹏,曾志磊,翟红,徐鸣明,张英英,余建萍,陈晓,彭丹,朱丹.新疆伊犁地区马的尼帕病毒感染情况研究[J].重庆医科大学学报,2008(S1):27-29. DOI：

LIU Yan-xi, et al. Research on Nipah virus infection in equine of Yili, Xinjiang Uygur Autonomous Region. [J]. Journal of Chongqing Medical University (S1):27-29(2008) DOI：

摘要

目的:探讨尼帕病毒（Nipah virus

NiV）在新疆伊犁地区马群中的自然感染情况。方法:采用尼帕病毒一步法实时定量逆转录聚合酶链反应（One step real time reverse transcriptase polymerase chain reaction

Real-time RT-PCR）检测120匹马外周血液单个核细胞（Peripheral blood mononuclear cells

PBMCs）中的NiVN基因片段

对阳性产物进行基因序列测定、同源性和氨基酸顺序分析。结果:所检测120匹马PBMCsNiVN基因片段阳性率为0%（0/120）。结论:本研究未发现新疆伊犁地区马中存在尼帕病毒自然感染。

Abstract

Objective:To investigate the epidemics of Nipah virus(NiV)in Yili

Xinjiang Uygur Autonomous Region. Methods:One step real time reverse transcriptase polymerase chain reaction(real-time RT-PCR)was performed to detect the NiV N in peripheral blood mononuclear cells from 120 equines. Gene sequence and amino acid sequence were analyzed for positive products.Results:The positive rate of NiV N in PBMCs from 120 equines was 0%(0/120).Conclusion:The investigation do not support that NiV infection exist in Yili

Xinjiang Uygur Autonomous Region.

关键词

尼帕病毒Real-time RT-PCR一步法Taqman探针

Keywords

Nipah virusReal-time RT-PCROne stepTaqman probe

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