Relationship between Wnt/β-Catenin signal pathway in gastric carcinoma cell line SGC-7901 and the expression of Caspase-8,Caspase-3 and Bcl-2

LING Kang; et al

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Relationship between Wnt/β-Catenin signal pathway in gastric carcinoma cell line SGC-7901 and the expression of Caspase-8,Caspase-3 and Bcl-2

更新时间：2024-01-17

- Relationship between Wnt/β-Catenin signal pathway in gastric carcinoma cell line SGC-7901 and the expression of Caspase-8,Caspase-3 and Bcl-2
- Journal of Chongqing Medical University Vol. 34, Issue 6, Pages: 690-693(2009)
- 作者机构：
  
  1. 重庆医科大学附属第一医院普外科
  2. 重庆医科大学公共卫生学院生殖生理教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.2
- Published：2009，
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[1]凌康,王颢.胃癌细胞株SGC-7901的Wnt/β-Catenin信号途径与Caspase-8、Caspase-3及Bcl-2的关系[J].重庆医科大学学报,2009,34(06):690-693.

LING Kang, et al. Relationship between Wnt/β-Catenin signal pathway in gastric carcinoma cell line SGC-7901 and the expression of Caspase-8,Caspase-3 and Bcl-2. [J]. Journal of Chongqing Medical University 34(6):690-693(2009)
[1]凌康,王颢.胃癌细胞株SGC-7901的Wnt/β-Catenin信号途径与Caspase-8、Caspase-3及Bcl-2的关系[J].重庆医科大学学报,2009,34(06):690-693. DOI：

LING Kang, et al. Relationship between Wnt/β-Catenin signal pathway in gastric carcinoma cell line SGC-7901 and the expression of Caspase-8,Caspase-3 and Bcl-2. [J]. Journal of Chongqing Medical University 34(6):690-693(2009) DOI：

摘要

目的:研究人胃癌细胞系SGC-7901中β-Catenin与原癌基因Bcl-2及凋亡基因Caspase-8、Caspase-3表达的关系

初步探索Wnt/β-Catenin通路在肿瘤发生过程中作用机制。方法:（1）采用RT-PCR法分别检测人胃癌细胞系SGC-7901受吲哚美辛干预组和未受干预组中的β-Catenin、Bcl-2、Caspase-8及Caspase-3mRNA的表达;（2）采用MTT法检测吲哚美辛抗癌效应。结果:（1）吲哚美辛干预组较未受干预组表现为伴随β-Catenin表达水平降低

Bcl-2表达下调

而Caspase-8及Caspase-3mRNA表达上调。（2）吲哚美辛对人胃癌细胞系SGC-7901抑制率呈浓度和时间依赖性。结论:胃癌的发生、发展可能是Wnt/β-Catenin通路功能亢进

进一步激活原癌基因Bcl-2

并抑制凋亡基因Caspase-8、Caspase-3

导致细胞增殖与凋亡失衡的结果。

Abstract

Objective:To study the regulatory mechanism of Wnt/β-Catenin passthway-influenced apoptosis of human gastric cancer line SGC-7901 by detecting the expression of β-Catenin mRNA

Bcl-2mRNA

Caspase-8mRNA and Caspase-3mRNA.Methods:(1) Expressions of β-Catenin

Bcl-2

Caspase-8 and Caspase-3mRNAs in groups with or without indomethacin induction were detected by RT-PCR.(2) Methyl thiazolyl tetrazolium(MTT) assay was used to measure the anticancer activity of indomethacin.Results:(1)As compared with groups without indomethacin induction

expression of β-Catenin mRNA and Bcl-2 mRNA were lower

however

increased expression of Caspase-8 and Caspase-3mRNA were found in SGC-7901 cells after treatment with indomethacin.(2) Indomethacin of 50 μmol/L at 24 h couldn’t induce apoptosis of gastric cancer cells.Indomethacin of 50μmol/L at 48 and 72 h and of 100μmol/L and 200μmol/L at 24、48 and 72 h could induce apoptosis of gastric cancer cells in a dose-and time-dependent manner.Conclusion:Gastric cancer cells may be caused by apoptosis inbalance through Wnt/β-Catenin passthway

which activates expression of proto-oncogene Bcl-2 and inhibites expressions of Caspase-8 and Caspase-3.

关键词

胃癌细胞凋亡Wnt/β-Catenin通路

Keywords

gastric cancerapoptosisWnt/β-Catenin passthway

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