Biological effect of maspin gene on VEGF in hepatocarcinoma cell MHCC-97

YAN Xiong

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Biological effect of maspin gene on VEGF in hepatocarcinoma cell MHCC-97

更新时间：2024-01-17

- Biological effect of maspin gene on VEGF in hepatocarcinoma cell MHCC-97
- Journal of Chongqing Medical University Vol. 34, Issue 6, Pages: 687-690(2009)
- 作者机构：
  
  重庆医科大学附属第一医院普外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.7
- Published：2009，
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[1]阎雄.抑癌基因maspin在肝癌细胞株MHCC-97中对VEGF的生物学作用[J].重庆医科大学学报,2009,34(06):687-690.

YAN Xiong. Biological effect of maspin gene on VEGF in hepatocarcinoma cell MHCC-97. [J]. Journal of Chongqing Medical University 34(6):687-690(2009)
[1]阎雄.抑癌基因maspin在肝癌细胞株MHCC-97中对VEGF的生物学作用[J].重庆医科大学学报,2009,34(06):687-690. DOI：

YAN Xiong. Biological effect of maspin gene on VEGF in hepatocarcinoma cell MHCC-97. [J]. Journal of Chongqing Medical University 34(6):687-690(2009) DOI：

摘要

目的:探讨抑癌基因maspin在肝癌细胞株MHCC-97中对血管内皮生长因子（Vascular endothelial growth factor

VEGF）的生物学作用及意义。方法:构建maspin/pEFIRES-N表达质粒

重组质粒转染后

采用RT-PCR及WesternBlot方法分别从mRNA和蛋白水平检测肝癌细胞株MHCC-97中maspin和VEGF转染前后的表达。结果:成功构建maspin/pEFIRES-N重组质粒

并稳定转染到肝癌细胞株MHCC-97中

显示转染maspin基因后在肝癌细胞株MHCC-97中maspin的mRNA和蛋白表达明显增强

而VEGF的mRNA和蛋白表达明显减弱（P<0.05）。结论:在肝癌细胞株MHCC-97中

maspin基因的过表达会降低VEGF的mRNA及蛋白表达

从而可能抑制新生血管形成

阻止肿瘤细胞的增生和转移。

Abstract

Objective:To explore the biological effect of maspin on VEGF(vascular endothelial growth factor

VEGF)in hepatocarcinoma cell MHCC-97 and discuss its significance.Methods:Maspin/pEFIRES-N expressing plasmid was constructed .The changes of maspin and VEGF in the levels of mRNA and protein in hepatocarcinoma cell MHCC-97 before and after the transfection and recombination were detected by RT-PCR and Western blotting.Results:The maspin/pEFIRES-N plasmid was constructed successfully

and was stably transferred into MHCC-97 cell.The expressions of maspin mRNA and protein increased in hepatocarcinoma cell MHCC-97 after the transfection of maspin/pEFIRES-N plasmid

while the expressions of VEGF mRNA and protein decreased

as compared with those after the transfection of pEFIRES-N plasmid(P<0.05).Conclusion:In hepatocarcinoma cell MHCC-97

maspin overexpression decreases the expressions of VEGF

which inhibits neovascularization

blocks the hyperplasia and metastasis of tumour cells.

关键词

maspin血管内皮生长因子肝癌细胞株MHCC-97

Keywords

maspinVEGFHepatocarcinomacellMHCC-97

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