Anti-apoptotic actions and mechanisms of ghrelin in cardiomyocytes after hypoxia/reoxygenation injury

FU Hang; et al

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Anti-apoptotic actions and mechanisms of ghrelin in cardiomyocytes after hypoxia/reoxygenation injury

更新时间：2024-01-17

- Anti-apoptotic actions and mechanisms of ghrelin in cardiomyocytes after hypoxia/reoxygenation injury
- Journal of Chongqing Medical University Vol. 34, Issue 6, Pages: 694-697(2009)
- 作者机构：
  
  重庆医科大学附属第一医院心内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R363
- Published：2009，
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[1]傅航,王华,黄德嘉.Ghrelin对缺氧/复氧心肌细胞凋亡的影响及其机制的研究[J].重庆医科大学学报,2009,34(06):694-697.

FU Hang, et al. Anti-apoptotic actions and mechanisms of ghrelin in cardiomyocytes after hypoxia/reoxygenation injury. [J]. Journal of Chongqing Medical University 34(6):694-697(2009)
[1]傅航,王华,黄德嘉.Ghrelin对缺氧/复氧心肌细胞凋亡的影响及其机制的研究[J].重庆医科大学学报,2009,34(06):694-697. DOI：

FU Hang, et al. Anti-apoptotic actions and mechanisms of ghrelin in cardiomyocytes after hypoxia/reoxygenation injury. [J]. Journal of Chongqing Medical University 34(6):694-697(2009) DOI：

摘要

目的:本实验研究Ghrelin对缺氧/复氧干预下心肌细胞凋亡的影响以及其具体的作用机制。方法:用体外培养的SD乳大鼠心肌细胞

建立缺氧（4h）/复氧（1h）模型。实验分4组:①对照组;②缺氧/复氧组（H/R）;③H/R+Ghrelin干预组;④H/R+Ghrelin+沃曼青霉素干预组。分别检测各组培养液中LDH浓度

Tunel法测定各组细胞凋亡率

免疫印迹法检测细胞内激酶磷酸化Akt的表达。结果:实验发现

与H/R组和H/R+Ghrelin+沃曼青霉素干预组相比H/R+Ghrelin组能明显减轻缺氧/复氧损伤所导致的LDH的漏出（30.00±6.60与78.00±4.20U/L;30.00±6.60与61.67±7.22U/L）

以及明显减低心肌细胞的凋亡率（0.0859±0.0303与0.3516±0.06329;0.0859±0.0303与0.3255±0.02258）（P<0.05）。同时H/R+Ghrelin干预组磷酸化Akt的表达明显增强。结论:Ghrelin具有减轻缺氧/复氧所引起的心肌细胞损伤

抑制心肌细胞凋亡的作用。与Ghrelin激活细胞内信号传递途径PI3K/Akt（磷脂酰肌醇3激酶/丝-苏氨酸激酶）有关。

Abstract

Objective:To explore the anti-apoptostic effect of Ghrelin in cardiomyocytes after Hypoxia/Reoxygenation injury and manifest the specific cellular singaling transduction pathway.Methods:Newborn SD rats’ hearts were isolated and cardiomyocytes were separated by trypsin digestion method .The primary cardiomyocytes were collected and incubated in vitro and randomly alloted to 4 groups(①Control;②H/R group;③H/R +Ghrelin group;④H/R+Ghrelin+Wortmannin group) after 5 days of cultivation.Hypoxia/ Reoxygenation injury was established by 4 hours of hyopxia and subsquently 1 hour of reoxygenation.Lactic dehydrogenase concentration

cell apoptotic rate and the expression of phosph-Akt were detected by Automatic Biochemistry Analytic Instrument

Tunel method and Western blotting method respectively.Results:Compared with H/R group and H/R+Ghrelin+Wortmannin group

the LDH concentration in media was significantly reduced and the myocardium apoptotic rate was pretty low in H/R+Ghrelin group.Ghrelin also increased phosph-Akt activity but this effect was abolished by applying Wortmannin simultaneously.Conclusions:Ghrelin provides concrete protection against Hypoxia/ Reoxygenation injury in the primary cultivated rat cardiomyocytes

which manifest dereasing LDH leaking

inhibitting cell apoptosis.Theantiapoptotic effect is partly mediated by the activation of the PI3K /Akt(Phosphatidylinositol-3 kinase/ Akt) pathway.

关键词

心肌细胞ghrelin缺氧/复氧凋亡PI3K/AKT

Keywords

cardiomyocyteghrelinHypoxia/Reoxygenation injuryapoptosisPI3K/AKT

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