Screening of mimic epitope for MUC1 based on prediction of the antigenic epitope

WANG Xiang-wei; et al

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Screening of mimic epitope for MUC1 based on prediction of the antigenic epitope

更新时间：2024-01-17

- Screening of mimic epitope for MUC1 based on prediction of the antigenic epitope
- Journal of Chongqing Medical University Vol. 34, Issue 9, Pages: 1148-1150(2009)
- 作者机构：
  
  第三军医大学新桥医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.1
- Published：2009，
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[1]王祥卫,叶钢,张远宁,易善红,金欢胜,方针强,贾维胜,周铮.基于抗原表位预测的MUC1模拟肽表位筛选[J].重庆医科大学学报,2009,34(09):1148-1150.

WANG Xiang-wei, et al. Screening of mimic epitope for MUC1 based on prediction of the antigenic epitope. [J]. Journal of Chongqing Medical University 34(9):1148-1150(2009)
[1]王祥卫,叶钢,张远宁,易善红,金欢胜,方针强,贾维胜,周铮.基于抗原表位预测的MUC1模拟肽表位筛选[J].重庆医科大学学报,2009,34(09):1148-1150. DOI：

WANG Xiang-wei, et al. Screening of mimic epitope for MUC1 based on prediction of the antigenic epitope. [J]. Journal of Chongqing Medical University 34(9):1148-1150(2009) DOI：

摘要

目的:预测粘蛋白1（Mucin1

MUC1）抗原的B细胞表位

从噬菌体随机多肽文库筛选及鉴定MUC1抗原的模拟表位。方法:对MUC1的二级结构及免疫原性进行分析

预测MUC1的抗原表位。利用纯化获得的Ma695抗体筛选噬菌体随机12肽库

夹心ELISA分析噬菌体克隆

测定阳性克隆DNA序列

竞争性抑制实验鉴定阳性噬菌体克隆。结果:MUC1存在有17个潜在的抗原表位区域。经3轮筛选

获得了14个阳性克隆

DNA序列分析并推导出其氨基酸序列:KHYDPFHHRMPQ

QADTARSVALAG

VPSKPDLHVRSI及MTPIHYWNHNRV;4个噬菌体展示肽克隆抑制率均在50%以上。结论:预测MUC1抗原B细胞表位

为进一步研究MUC1结构和功能奠定了基础。所得序列KHYDPFHHRMPQ

QADTARSVALAG

VPSKPDLHVRSI及MTPIHYWNHNRV能模拟MUC1抗原表位。

Abstract

Objective:To predict the B cell epitope for MUC1 antigene and screen the mimic epitope of MUC1 from random phage dispay peptide library. Methods:In order to predict the B cell epitope for MUC1 antigen

the secondary structure and antigenicity was analysed with various methods. The purified Ma695 Ab was used to screen in phage random 12 peptide library. The positive clones were identified by sandwich ELISA and competitive inhibiton assay. Results:17 distinct antigenic epitope regions in MUC1 were identified by computation. 14 positive clones were acquired after 3 rounds of screening. Amino acid sequences deduced from DNA sequences showed four different sequences:KHYDPFHHRMPQ

QADTARSVALAG

VPSKPDLHVRSI and MTPIHYWNHNRV. The inhibitory assay showed that the 4 mimic epitope peptides displaying on the phage surface could effectively inhibit the combination of antibody with antigen and the inhibitory rates of each mimic epitope were 50% higher than that in the controls. Conclusion: Prediction of the B cell epitope for MUC1 can provide a basic clues for studies on structure and function of MUC1. The results indicated that KHYDPFHHRMPQ

QADTARSVALAG

VPSKPDLHVRSI and MTPIHYWNHNRV are the mimotopes which could mimic the epitope of MUC1.

关键词

MUC1抗原模拟表位噬菌体随机12肽库

Keywords

Mucin 1Mimic epitopePhage display random 12 peptide library

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