Effect of survivin expression on the maturation of mouse marrow dendritic cells

LI Yan-min; et al

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Effect of survivin expression on the maturation of mouse marrow dendritic cells

更新时间：2024-01-17

- Effect of survivin expression on the maturation of mouse marrow dendritic cells
- Journal of Chongqing Medical University Vol. 34, Issue 9, Pages: 1144-1147(2009)
- 作者机构：
  
  重庆医科大学附属第一医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392
- Published：2009，
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[1]黎衍敏,苟欣,何卫阳,王海峰.Survivin表达对鼠髓样树突状细胞成熟的影响[J].重庆医科大学学报,2009,34(09):1144-1147.

LI Yan-min, et al. Effect of survivin expression on the maturation of mouse marrow dendritic cells. [J]. Journal of Chongqing Medical University 34(9):1144-1147(2009)
[1]黎衍敏,苟欣,何卫阳,王海峰.Survivin表达对鼠髓样树突状细胞成熟的影响[J].重庆医科大学学报,2009,34(09):1144-1147. DOI：

LI Yan-min, et al. Effect of survivin expression on the maturation of mouse marrow dendritic cells. [J]. Journal of Chongqing Medical University 34(9):1144-1147(2009) DOI：

摘要

目的:探讨survivin的表达变化对小鼠骨髓来源树突状细胞分化成熟的影响。方法:无菌条件下取小鼠的骨髓细胞作为树突状前体细胞

用粒细胞巨噬细胞集落刺激因子（Granulocyte macrophage colony-stimulating factor

GM-CSF）和白细胞介素4（Interleukin 4

IL-4）的培养基定向诱导树突状前体细胞分化成未成熟树突状细胞（Immatureden driticcells

iDC）

用GM-CSF

IL-4及肿瘤坏死因子-α（Tumor necrosis factor-alpha

TNF-α）定向诱导树突状前体细胞分化成成熟树突状细胞（Matureden driticcells

mDC）:运用半定量RT-PCR

Westernblot比较树突状前体细胞、iDC与mDC中survivinm RNA、survivin蛋白的含量变化。结果:RT-PCR检测结果显示

树突状前体细胞survivinmRNA表达值为（0.735±0.081）

iDC为（0.596±0.073

P<0.05）

mDC为（0.393±0.052

P<0.01）;Westernblot检测结果显示:树突状前体细胞survivin蛋白的表达值为（0.767±0.045）

iDC的表达值为（0.396±0.016

P<0.05）

mDC的表达值为（0.168±0.009

P<0.01）。结论:survivin在树突状前体细胞中高表达

在iDC与mDC中表达量略低;且survivin在iDC中的表达量高于mDC

这个结论为我们的后续实验提供了理论基础。

Abstract

Objective:To study the effect of survivin expression on the mature process of dendritic cells from mouse bone marrow. Methods:The cells in marrow as the precurosor of the dendritic cells were taken from mouse in bioclean condition. Then the iDC were induced by the precurosor of DCs with GM-CSF and IL-4 in medium. ThemDC were induced by the precurosor of DCs with GM-CSF

IL-4

and TNF-α in medium. Survivin was compared by RT-PCR

and Western-bolt among these three stages. Results:The RT-PCR showed that the expression ratios of survivin mRNA among the precurosor of DCs

iDC

and mDC were(0.735±0.081)

(0.596±0.073

P<0.05)

and(0.393±0.052

P<0.01). Respectively

the Westernblot showed that the expression ratios of survivin proteinum among the above-mentioned DC’s three stages were(0.767±0.045)

(0.396±0.016

P<0.05)

and(0.168±0.009

P<0.01). Conclusion: Compared with thesurvivin in iDC and mDC

survivin in the precurosor of DCs was expressed more highly;And it was more highly expressed in iDC than in mDC. This conclusion is providing fundamental basis for our follow-up experiments.

关键词

树突状细胞Survivin分化

Keywords

dendritic cellsSurvivinDifferentiate

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