An on-the-spot method for rapid determination of quality of artemisiae annuae,herba

ZUO Ju-bo; et al

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An on-the-spot method for rapid determination of quality of artemisiae annuae,herba

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- An on-the-spot method for rapid determination of quality of artemisiae annuae,herba
- Journal of Chongqing Medical University Vol. 34, Issue 2, Pages: 206-209(2009)
- 作者机构：
  
  1. 重庆医科大学药学院药物分析教研室
  2. 重庆医科大学医药研究所
- 作者简介：
- 基金信息：
- DOI：
  CLC： R284
- Published：2009，
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[1]左巨波,代广会,杨俊卿,母昭德,尚京川.现场快速检测青蒿药材质量的方法研究[J].重庆医科大学学报,2009,34(02):206-209.

ZUO Ju-bo, et al. An on-the-spot method for rapid determination of quality of artemisiae annuae,herba. [J]. Journal of Chongqing Medical University 34(2):206-209(2009)
[1]左巨波,代广会,杨俊卿,母昭德,尚京川.现场快速检测青蒿药材质量的方法研究[J].重庆医科大学学报,2009,34(02):206-209. DOI：

ZUO Ju-bo, et al. An on-the-spot method for rapid determination of quality of artemisiae annuae,herba. [J]. Journal of Chongqing Medical University 34(2):206-209(2009) DOI：

摘要

目的:建立一种简便、快速、准确、灵敏的现场测定青蒿药材中青蒿素含量的检测方法。方法:以索氏提取-高效液相色谱法为对照

采用超声波提取-紫外分光度法进行现场快速检测青蒿药材质量。结果:得出现场快速检测青蒿素的含量关系式为:MRD（mg/g）=（A×V×2.2984）（/As×3）+1.2067。结论:超声波提取-紫外分光度法测定青蒿质量

简化了操作程序

缩短了提取时间

在1.5h内就可完成青蒿素的含量测定

是目前快检速测青蒿药材质量较理想的方法。

Abstract

Objective:To establish a simple

fast

exact and sensitive method to determine the quality of arteannuin on the spot.Methods:In contrast to the results based on HPLC in the aid of soxhlet extraction

an on-the-spot determination of Artemisinin by UV aided by ul-trasonic extraction was proposed.Results:The content of Artemisinin extracted with on-the-spot method for rapid determination is com-puted by equation:MRD(mg.g-1)=(A×V×2.2984)(/As×3)+1.2067.Conclusion:This method with ultrasonic extraction of artean-nuin and UV determination simplified the procedure and saved work time.It proved to be an satisfactory method for determination of arteannuin.

关键词

青蒿青蒿素快速检测紫外分光光度法高效液相色谱法

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