Separation and determination of toosendanin in the bark of melia toosendan sieb.et zuccc.by improved transonic extraction and HPLC separation

LIU Xiao-ling; et al

您当前的位置：

首页 >

文章列表页 >

Separation and determination of toosendanin in the bark of melia toosendan sieb.et zuccc.by improved transonic extraction and HPLC separation

更新时间：2024-01-17

- Separation and determination of toosendanin in the bark of melia toosendan sieb.et zuccc.by improved transonic extraction and HPLC separation
- Journal of Chongqing Medical University Vol. 34, Issue 2, Pages: 203-205(2009)
- 作者机构：
  
  重庆医科大学临床检验诊断学教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R284
- Published：2009，
扫描看全文
[1]刘小玲,颜伟伟,王进,张彦,易钢,何於娟.改良超声波萃取-高效液相色谱法分离、检测川楝树皮提取物中川楝素[J].重庆医科大学学报,2009,34(02):203-205.

LIU Xiao-ling, et al. Separation and determination of toosendanin in the bark of melia toosendan sieb.et zuccc.by improved transonic extraction and HPLC separation. [J]. Journal of Chongqing Medical University 34(2):203-205(2009)
[1]刘小玲,颜伟伟,王进,张彦,易钢,何於娟.改良超声波萃取-高效液相色谱法分离、检测川楝树皮提取物中川楝素[J].重庆医科大学学报,2009,34(02):203-205. DOI：

LIU Xiao-ling, et al. Separation and determination of toosendanin in the bark of melia toosendan sieb.et zuccc.by improved transonic extraction and HPLC separation. [J]. Journal of Chongqing Medical University 34(2):203-205(2009) DOI：

摘要

目的:建立改良的快速分离、检测川楝树皮提取物中川楝素（Toosendanin

TSN）的超声波萃取-高效液相色谱法。方法:采用超声波乙醇提取-氯仿萃取制备川楝树皮提取物

高效液相色谱法（High performance liquid chromatography

HPLC）分析提取物中的TSN含量

以乙腈-水（45∶55）为流动相

检测波长215nm

流速1ml/min

柱温:25℃

色谱柱为XDBC18柱（4.6×250mm

5μm）。结果:TSN在4.64～88.2μg/ml浓度范围内有良好的线性关系（r=0.9996）

测定方法平均加标回收率为100.83%

精密度试验RSD为1.42%

稳定性试验RSD为2.26%。提取物中TSN经HPLC分离提纯后纯度达96.32%。结论:本方法简便、快速、准确

所得结果稳定、重复性好

可作为实验室少量制备和鉴定TSN纯品的方法。

Abstract

Objective:To establish an improved transonic extraction-high performance liquid chromatography(HPLC)method for separating and detecting toosendanin(TSN)in Melia toosendanin extractive.Methods:Transonic alcohol-chloroform extraction method was used to extract TSN from the bark of Melia toosendanin sieb.et zuccc.The content determination of TSN was performed on an Agilent XDB C18 Column(4.6×250 mm

5μm).Elution was performed using 45%acetonitrile-water solution as the mobile phase at a1flow rate of 1ml/min.Detection was performed at 215 nm wavelength.Results:The linear regression equation for peak area-TSN concentration(4.64～88.2μg/ml)was:A=7.616 2C-2.567 7

r=0.999 6;mean recovery of standard substance was 100.83%;RSD of precision test was 1.42%;RSD of stability test was 2.26%.Conclusion:The method is simple

rapid

stable and roducible

which can be used as a method for preparation and identification TSN of Melia toosendanin extractive in laboratory.

关键词

川楝素超声波萃取高效液相色谱法

Keywords

ToosendaninTransonic extractionHigh performance liquid chromatograph(yHPLC)

references

Views

228

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰