Effects of granulocyte-macrophage colony-stimulating factor on the expression of cysteine-cysteine chemokine receptor 2 in THP-1 monocytes

LI Jiong; et al

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Effects of granulocyte-macrophage colony-stimulating factor on the expression of cysteine-cysteine chemokine receptor 2 in THP-1 monocytes

更新时间：2024-01-17

- Effects of granulocyte-macrophage colony-stimulating factor on the expression of cysteine-cysteine chemokine receptor 2 in THP-1 monocytes
- Journal of Chongqing Medical University Vol. 34, Issue 6, Pages: 706-709(2009)
- 作者机构：
  
  重庆医科大学附属第二医院血管外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R654.3
- Published：2009，
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[1]李炯,罗文军,陈以宽.粒-巨细胞集落刺激因子对单核细胞表达CCR2的影响[J].重庆医科大学学报,2009,34(06):706-709.

LI Jiong, et al. Effects of granulocyte-macrophage colony-stimulating factor on the expression of cysteine-cysteine chemokine receptor 2 in THP-1 monocytes. [J]. Journal of Chongqing Medical University 34(6):706-709(2009)
[1]李炯,罗文军,陈以宽.粒-巨细胞集落刺激因子对单核细胞表达CCR2的影响[J].重庆医科大学学报,2009,34(06):706-709. DOI：

LI Jiong, et al. Effects of granulocyte-macrophage colony-stimulating factor on the expression of cysteine-cysteine chemokine receptor 2 in THP-1 monocytes. [J]. Journal of Chongqing Medical University 34(6):706-709(2009) DOI：

摘要

目的:探讨粒-巨细胞集落刺激因子（Granulocyte-macrophage colony-stimulating factor

GM-CSF）对体外培养的人单核细胞株THP-1表达半胱氨酸-半胱氨酸趋化因子受体2（Cysteine-cysteine chemokine receptor2

CCR2）mRNA和蛋白的影响。方法:以不同浓度的GM-CSF处理THP-1细胞

应用半定量RT-PCR方法检测CCR2mRNA表达

并应用Western蛋白印迹法进一步检测CCR2蛋白的表达。结果:对照组THP-1细胞表达较低水平的CCR2mRNA和蛋白

经GM-CSF刺激后各实验组CCR2mRNA和蛋白表达随GM-CSF浓度和时间的增加而增强。结论:GM-CSF可以刺激THP-1细胞CCR2mRNA和蛋白的表达

且其增加程度与GM-CSF刺激浓度及刺激时间呈正性相关。

Abstract

Objective:To investigate the effects of granulocyte-macrophage colony-stimulating factor on expressions of CC chemokine receptor 2(CCR2) mRNA and protein in cultured human THP-1 cell lines.Methods:THP-1 cell was stimulated by GM-CSF of different concentrations and time intervals.The mRNA expression of CCR2 was tested by RT-PCR

The protein expression of CCR2 was analyzed by Western blotting.Results:RT-PCR and Western blotting showed that THP-1 cell in control group expressed a low-level of CCR2 mRNA and protein.After stimulated by GM-CSF

the expression of CCR2 mRNA and protein increased in a concentration-and time-dependent manner.Conclusion:GM-CSF could stimulate THP-1 cell to express CCR2 mRNA and protein

in an concentration-and time-dependent manner.

关键词

粒-巨细胞集落刺激因子单核细胞CC趋化因子受体2

Keywords

Granulocyte-macrophage colony-stimulating factormonocytesCysteine-cysteine chemokine receptor 2

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