The influence of BMP<sub>9</sub> in myocardiocytes differentiation of stem cell in vitro

TAN Jin-tong; et al

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The influence of BMP₉ in myocardiocytes differentiation of stem cell in vitro

更新时间：2024-01-17

- The influence of BMP₉ in myocardiocytes differentiation of stem cell in vitro
- Journal of Chongqing Medical University Vol. 34, Issue 6, Pages: 672-676(2009)
- 作者机构：
  
  重庆医科大学儿童医院心血管内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329
- Published：2009，
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[1]谭金童,陈沅,向平.BMP_9对C_3H10T_(1/2)干细胞向心肌样细胞分化的影响[J].重庆医科大学学报,2009,34(06):672-676.

TAN Jin-tong, et al. The influence of BMP₉ in myocardiocytes differentiation of stem cell in vitro. [J]. Journal of Chongqing Medical University 34(6):672-676(2009)
[1]谭金童,陈沅,向平.BMP_9对C_3H10T_(1/2)干细胞向心肌样细胞分化的影响[J].重庆医科大学学报,2009,34(06):672-676. DOI：

TAN Jin-tong, et al. The influence of BMP₉ in myocardiocytes differentiation of stem cell in vitro. [J]. Journal of Chongqing Medical University 34(6):672-676(2009) DOI：

摘要

目的:研究骨形态形成蛋白9（Bone morphogenetic proteins9

BMP9）在C3H10T1/2干细胞向心肌细胞分化过程中的影响。方法:以高滴度（2.67ml/L）pAdEasy-BMP9重组腺病毒质粒转染C3H10T1/2干细胞

1周后应用RT-PCR方法及激光共聚焦方法分别检测C3H10T1/2干细胞中心肌特异转录因子及特异性蛋白的表达变化。2周后应用电子显微镜观察细胞超微结构的改变。结果:C3H10T1/2干细胞经pAdEasy-BMP9重组腺病毒质粒诱导1周后

细胞体积明显变大

排布走向趋于一致

细胞间连接紧密

折光性增强;细胞中开始出现心肌特异性转录因子NKx2.5、GATA-4、MEF2C及心肌特异性表达蛋白连接蛋白43（Connex-in43

Cx43）和心肌特异性肌钙蛋白T（Cardiac isoform of TropninT

cTnT）的表达;出现心肌特异性超微结构肌丝和闰盘。结论:BMP9可影响体外培养的C3H10T1/2干细胞定向分化为心肌样细胞。

Abstract

Objective:ToexploreinfluenceofBMP9inmyocardiocytesdifferentiationof stem cell Invitro.Methods:TheC3H10T1/2stem cell were transfected with 2.67 ml/L pAdEasy-BMP9 Ad-GFP plasmid for 1 week and 2 weeks .The specific transcription factors of cardiomyocytes were detected in stem cells during differentiation with plasmid transfection by RT-PCR and the specific proteins in cardiomy-ocytes were detected by laser confocal microsopy after 1 week;the change of cells ultramicro structure were detected by electron micro-scope after 2 weeks.Results:Cells volume are increscent obviously

cells trend become unanimous

the connection between cells are compact

refractivity of cells enhance conspicuously after induction for 1 week.The expression of NKx 2.5

GATA-4

MEF2C could be de-tected in C3H10T1/2 stem cells with pAdEasy-BMP9 Ad-GFP plasmid tranfection.Cx43 and cTnT can be detected with plasmid tranfec-tion.Cardiomyocytes ultramicro structure can be detected after induction for 2 weeks.Conclusion:BMP9 makes a very important influ-ence in C3H10T1/2 stem cells targeted differentiation into cardiomyocytes。

关键词

BMP9C3H10T1/2干细胞分化心肌细胞

Keywords

BMP9C3H10T1/2stemcelldifferentiationcardiomyocytes

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The influence of BMP9 in myocardiocytes differentiation of stem cell in vitro

DOI：

摘要

Abstract

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Keywords

references

The influence of BMP₉ in myocardiocytes differentiation of stem cell in vitro