The expression and significance of Dicer in gastric cell lines and gastric cancer tissue

NIE Na; et al

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The expression and significance of Dicer in gastric cell lines and gastric cancer tissue

更新时间：2024-01-17

- The expression and significance of Dicer in gastric cell lines and gastric cancer tissue
- Journal of Chongqing Medical University Vol. 34, Issue 6, Pages: 668-671(2009)
- 作者机构：
  
  重庆医科大学附属第二医院消化内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.2
- Published：2009，
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[1]聂娜,吴小翎,刘波.Dicer在胃癌细胞和胃癌组织的表达及意义[J].重庆医科大学学报,2009,34(06):668-671.

NIE Na, et al. The expression and significance of Dicer in gastric cell lines and gastric cancer tissue. [J]. Journal of Chongqing Medical University 34(6):668-671(2009)
[1]聂娜,吴小翎,刘波.Dicer在胃癌细胞和胃癌组织的表达及意义[J].重庆医科大学学报,2009,34(06):668-671. DOI：

NIE Na, et al. The expression and significance of Dicer in gastric cell lines and gastric cancer tissue. [J]. Journal of Chongqing Medical University 34(6):668-671(2009) DOI：

摘要

目的:探讨Dicer在胃癌（Gastric carcinoma）细胞和胃癌组织中的表达及意义。方法:应用Western-Blot和逆转录-聚合酶链反应（Reverse transcriptase polymerase chain reaction

RT-PCR）检测正常胃粘膜细胞株GES-1、人胃癌细胞株SGC-7901

25例胃癌手术标本及癌旁配对组织Dicer的含量。结果:1.Western-Blot和RT-PCR结果表明Dicer在人胃癌细胞株SGC-7901的表达水平明显低于正常胃粘膜细胞株GES-1（P<0.05）

在胃癌手术标本组织的表达水平明显低于癌旁配对组织（P<0.05）;2.Dicer表达水平与胃癌分化程度有关。低分化组低于中分化组（P<0.05）

中分化组低于高分化组（P<0.05）。结论:在胃癌细胞株和胃癌组织中Dicer表达明显降低并且Dicer表达与胃癌分化程度相关。

Abstract

Objective:To detect the expression and significance of Dicer in gastric cell lines and gastric cancer tissue.Methods:Western-Blot and reverse transcriptase polymerase chain reaction(RT-PCR) were used to detect the expression of Dicer in normal gastric mucosa cell line GES-1

human gastric cancer cell line SGC-7901

25 surgical removed gastric cancer tissue and paired para-tumor tissue.Results:1.The expression of Dicer in human gastric cancer cell line SGC-7901 was lower than that in normal gastric mucosa cell line GES-1(P<0.05)

the expression of Dicer in gastric cancer tissue was lower than that in complete sequence tissue(P<0.05);2.The expression of Dicer is correlated with differentiation degrees of gastric cancer.The expression of Dicer in poorly differentiation group is lower than that in moderately differentiation group(P<0.05)

and the expression of Dicer in moderately differentiation group is lower than that in well differentiation group(P<0.05).Conclusion:The expression of Dicer is obvious descend in gastric cancer tissue and gastric cancer cell lines and correlated with differentiation degrees of gastric cancer.

关键词

Dicer胃癌逆转录-聚合酶链反应

Keywords

Dicergastric carcinomaRT-PCR

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Related Institution

重庆医科大学第二临床学院消化内科

重庆医科大学附属第一医院消化内科

Department of Gastroenterology,the First Affiliated Hospital,Chongqing Medical University

Department of Digestive Diseases,the First Affiliated Hospital,Chongqing Medical University

Department of Surgery,the First Affiliated Hospital,Chongqing Medical University

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