Study on the dynamic distribution of [<sup>14</sup>C]benzo（a）pyrene in the corpus striatum

YUAN Hai-yan; et al

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Study on the dynamic distribution of [¹⁴C]benzo（a）pyrene in the corpus striatum

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- Study on the dynamic distribution of [¹⁴C]benzo（a）pyrene in the corpus striatum
- Journal of Chongqing Medical University Vol. 34, Issue 6, Pages: 677-679(2009)
- 作者机构：
  
  1. 重庆医科大学公共卫生学院劳动卫生与环境卫生教研室
  2. 泸州医学院公共卫生系劳动卫生教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R114
- Published：2009，
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[1]袁海艳,汤艳,程淑群,涂白杰.[~(14)C]苯并[a]芘在SD大鼠纹状体的动态分布研究[J].重庆医科大学学报,2009,34(06):677-679.

YUAN Hai-yan, et al. Study on the dynamic distribution of [¹⁴C]benzo（a）pyrene in the corpus striatum. [J]. Journal of Chongqing Medical University 34(6):677-679(2009)
[1]袁海艳,汤艳,程淑群,涂白杰.[~(14)C]苯并[a]芘在SD大鼠纹状体的动态分布研究[J].重庆医科大学学报,2009,34(06):677-679. DOI：

YUAN Hai-yan, et al. Study on the dynamic distribution of [¹⁴C]benzo（a）pyrene in the corpus striatum. [J]. Journal of Chongqing Medical University 34(6):677-679(2009) DOI：

摘要

目的:用r测量方法和光镜放射自显影研究[14C]苯并[a]芘（[14C]Benzo（a）pyrene

[14C]BaP）在大鼠纹状体的动态分布

探讨BaP的神经毒性及其作用机制。方法:100只SD大鼠随机分为对照组和实验组

实验组尾静脉注射[14C]BaP3.7×105Bq/kg

对照组注射相同剂量的生理盐水。每组按照染毒后处死时间的不同

又分为1h、1d、2d、3d、7d共5个时间点

观察大鼠一般情况以及测定脏器系数

采用r测量方法和光镜放射自显影法观察[14C]BaP在纹状体的分布及其银粒密度。结果:BaP急性染毒后大鼠出现了一般情况的改变和脏器系数下降。r测量结果:纹状体每克脑组织含放射性占注射剂量的百分比（%ID/g）在第1d和第2d明显高于海马、大脑皮层（P<0.05）;放射自显影结果:纹状体银粒在第1d达高峰

第2d显著减少

染毒后第7d仍可检测到银粒。结论:BaP能透过血脑屏障到达脑组织

主要分布和贮存于纹状体

具有一定的中枢神经毒性。

Abstract

Objective:To study the dynamic distribution of BaP in the corpus striatum by r-counting and light microscopic autoradiography

then to explore the neurotoxic mechanism of BaP.Methods:100 SD male rats were divided into control group(n=40)and test group(n=60)

experimental animals were given a single intravenous injection of 3.7×105 Bq/kg of 14CBaP while the same doses of Normal Saline were given to the control group.The rats were sacrificed at 1h

1 d

2 d

3 d and 7 d after the administration of radiolabelled BaP.During the experiment

some toxicological symptoms were observed and the ratios of brain-weight/body-weight were detected.Light microscopic autoradiography and r-counting were used to observe the dynamic distribution of BaP in the corpus striatum.Results:The change of toxicological symptoms are observed and the decrease ratios of brain-weight/body-weight are detected.R-counting shows that the percentage dose/g of 14CBaP in striatum is significant higher than in hippocampus and cortex at 1d and 2 d after administration.Light microscopic autoradiography shows that the silver granules in striatum reach the peak in 1d and sharp decrease in 2 d

which can be found even at 7d.Conclusion:BaP can penetrate the blood-brain barrier and distribute in corpus striatum

inducing CNS toxicity in SD rat.

关键词

苯并[a]芘光镜放射自显影r测量法纹状体

Keywords

Benzo(a)pyreneLight microscopic autoradiographyR-countingCorpus striatum

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Effects of benzo(a) pyrene exposure on morphology of the nervous system and its mechanism

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Study on the dynamic distribution of [14C]benzo（a）pyrene in the corpus striatum

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摘要

Abstract

关键词

Keywords

references

Study on the dynamic distribution of [¹⁴C]benzo（a）pyrene in the corpus striatum