Transgene expression in intervertebral disc cells in vivo mediated by microbubble-enhanced ultrasound gene therapy

YAN Zheng-jian; et al

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Transgene expression in intervertebral disc cells in vivo mediated by microbubble-enhanced ultrasound gene therapy

更新时间：2024-01-17

- Transgene expression in intervertebral disc cells in vivo mediated by microbubble-enhanced ultrasound gene therapy
- Journal of Chongqing Medical University Vol. 34, Issue 6, Pages: 684-686(2009)
- 作者机构：
  
  1. 重庆医科大学附属第二医院骨科
  2. 重庆医科大学试验检验学教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R450;R681.5
- Published：2009，
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[1]晏铮剑,邓忠良,罗进勇,赵迎泽,施琼.超声微泡介导双报告基因在椎间盘髓核中表达的研究[J].重庆医科大学学报,2009,34(06):684-686.

YAN Zheng-jian, et al. Transgene expression in intervertebral disc cells in vivo mediated by microbubble-enhanced ultrasound gene therapy. [J]. Journal of Chongqing Medical University 34(6):684-686(2009)
[1]晏铮剑,邓忠良,罗进勇,赵迎泽,施琼.超声微泡介导双报告基因在椎间盘髓核中表达的研究[J].重庆医科大学学报,2009,34(06):684-686. DOI：

YAN Zheng-jian, et al. Transgene expression in intervertebral disc cells in vivo mediated by microbubble-enhanced ultrasound gene therapy. [J]. Journal of Chongqing Medical University 34(6):684-686(2009) DOI：

摘要

目的:探索超声微泡能否介导外源性基因在椎间盘髓核中表达。方法:40只SD大鼠被随机分为4组。第1组仅于髓核内注入含双报告基因的质粒;第2组为在第1组基础上加超声照射;第3组同时注入含双报告基因的质粒和微泡的混悬液;第4组为在第3组基础上加超声照射。每只大鼠未接受手术和照射的第4、5椎间盘作为对照。一周后处死大鼠取出全部椎间盘做荧光素酶检测。结果:第4组（96.7×103±72.3×103）荧光素酶强度最高

与第1组（8.7×103±7.2×103）、第2组（3.1×103±2.4×103）、第3组（47.5×103±43.3×103）比较

差异有统计学意义（P<0.01）。结论:超声微泡介导的基因转染系统能促进外源基因在髓核中的高表达

为今后椎间盘病变的基因治疗提供了方法和依据。

Abstract

Objective:To evaluate the effect of ultrasound contrast agent on expression of plasmid in intervertebral disc of Sprague-Dawley rats.Methods:Forty adult Sprague-Dawley rats were studied.The rats were randomly divided into 4 groups.Two different reporter plasmid DNA encoding green fluorescent protein(GFP) and firefly luciferase were used.Plasmid DNA was mixed with ultrasonography contrast agent(microbubbles) and injected into coccygeal intervertebral discs of Sprague-Dawley rats.The ultrasound was irradiated on the surface of injected discs.Rats were killed 7 days after gene transduction.Harvested nucleus pulposus tissues were used to evaluate gene expression.The intact discs were used as a control.Results:Significant luciferase activity was observed in group 4;less in the other groups and none in the control.Conclusion:Ultrasound with microbubble contrast agent induced efficient gene transfer to interverbetebral disc of rats after local injection of plasmid.

关键词

基因治疗超声微泡椎间盘

Keywords

gene therapyultrasoundmicrobubbleIntervertebral disc

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