Effect of recombined RNAi plasmids on apoptosis of cisplatin-resistant cell line Acc-3/CDDP

ZHANG Fu-Jun; et al

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Effect of recombined RNAi plasmids on apoptosis of cisplatin-resistant cell line Acc-3/CDDP

更新时间：2024-01-17

- Effect of recombined RNAi plasmids on apoptosis of cisplatin-resistant cell line Acc-3/CDDP
- Journal of Chongqing Medical University Vol. 34, Issue 6, Pages: 680-683(2009)
- 作者机构：
  
  1. 重庆医科大学附属第一医院口腔颌面外科
  2. 重庆医科大学附属口腔医院口腔颌面外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.8
- Published：2009，
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[1]张福军,季平,杨凯,张劲松.Bcl-2重组干扰质粒促进多药耐药Acc-3/CDDP细胞凋亡实验研究[J].重庆医科大学学报,2009,34(06):680-683.

ZHANG Fu-Jun, et al. Effect of recombined RNAi plasmids on apoptosis of cisplatin-resistant cell line Acc-3/CDDP. [J]. Journal of Chongqing Medical University 34(6):680-683(2009)
[1]张福军,季平,杨凯,张劲松.Bcl-2重组干扰质粒促进多药耐药Acc-3/CDDP细胞凋亡实验研究[J].重庆医科大学学报,2009,34(06):680-683. DOI：

ZHANG Fu-Jun, et al. Effect of recombined RNAi plasmids on apoptosis of cisplatin-resistant cell line Acc-3/CDDP. [J]. Journal of Chongqing Medical University 34(6):680-683(2009) DOI：

摘要

目的:探讨表达重组干扰质粒沉默Bcl-2基因后对多药耐药Acc-3/CDDP细胞凋亡的影响。方法:按siRNA设计原则构建针对Bcl-2基因表达重组RNA干扰质粒

脂质体转染多药耐药Acc-3/CDDP细胞后分别行MTT试验、电子显微镜检查、AO/EB荧光染色法检测

观察不同条件处理后各组细胞的凋亡情况有无差别。结果:MTT试验见随着时间的延长

基因干扰组的OD值进行性降低

降低幅度在转染48h时最大

至72h时曲线明显较前变缓。电子显微镜检查结果显示

基因干扰组从转染后24～72h出现进行性加重的细胞凋亡改变

各期凋亡细胞清晰典型。荧光染色法检测细胞凋亡结果显示

基因干扰组能明显促进Acc-3/CDDP细胞凋亡（P<0.05）。结论:表达重组干扰质粒能有效地沉默多药耐药Acc-3/CDDP细胞中Bcl-2基因表达

促进细胞凋亡。

Abstract

Objective:To investigate the effect of the silence of Bcl-2 oncogene on the apoptosis of cisplatin-resistant cell line Acc-3/CDDP.Methods:The recombined RNAi plasmids for Bcl-2 oncogene were constituted by the four successive steps-designing of Oligo DNAs

and were transfected into Acc-3/CDDP cells by culturing together.The apoptosis of each cells were evaluated by MTT test

electron microscope

and AO/EB fluorescent dyes respectively.Results:The OD value of RNAi group decreased with time last by MTT test

and dropped to the lowest at 48 h.The apoptosis of RNAi group were observable through electron microscope and fluorescent dyes.The apoptosis rate increased with time from 24 to 72 h after transfection.The apoptosis on each cell cycle were clear and classical.Conclusion:Recombined interfering plasmids can effectively silence the expression of Bcl-2 oncogenes

and the apoptosis of cisplatin-resistant cell line Acc-3/CDDP cells can be enhanced.

关键词

基因干扰基因沉默细胞凋亡细胞周期

Keywords

RNA interferenceGene silencecell apoptosiscell cycle

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