Effect of curcumin on the activity of ATPase in U-251 cells

CHEN Song; et al

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Effect of curcumin on the activity of ATPase in U-251 cells

更新时间：2024-01-17

- Effect of curcumin on the activity of ATPase in U-251 cells
- Journal of Chongqing Medical University Vol. 34, Issue 6, Pages: 665-668(2009)
- 作者机构：
  
  重庆医科大学附属第二医院神经外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.41;R285.5
- Published：2009，
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[1]陈松,程远.姜黄素对胶质瘤细胞ATP酶的影响[J].重庆医科大学学报,2009,34(06):665-668.

CHEN Song, et al. Effect of curcumin on the activity of ATPase in U-251 cells. [J]. Journal of Chongqing Medical University 34(6):665-668(2009)
[1]陈松,程远.姜黄素对胶质瘤细胞ATP酶的影响[J].重庆医科大学学报,2009,34(06):665-668. DOI：

CHEN Song, et al. Effect of curcumin on the activity of ATPase in U-251 cells. [J]. Journal of Chongqing Medical University 34(6):665-668(2009) DOI：

摘要

目的:探讨姜黄素（curcumin）对人脑胶质瘤U-251细胞ATP酶的影响及诱导胶质瘤细胞凋亡的机制。方法:分别用20、40、80和100μmol/L姜黄素处理U-251细胞24h

用MTT法测定姜黄素对U-251细胞增殖的抑制作用;流式细胞仪检测细胞凋亡

比色法测定ATP酶活力。结果:姜黄素抑制U251细胞的增殖;诱导U251细胞凋亡;姜黄素明显使细胞质膜ATP酶活力明显降低。结论:姜黄素通过抑制细胞质膜ATP酶活性可诱导胶质瘤细胞凋亡和抑制增殖。

Abstract

Objective:To study the effects of curcumin on the activity of ATPase and the mechanism of apoptosis in U-251 cell.Methods:U-251 cells were treated with 20

100μmol/L curcumin for 24 h and the growth inhibition rates of U-251 cells were measured with MTT method.Cell apoptosis was inspected with flow cytometry(FCM).The activities of ATPase were determined by colorimetry method.Results:Curcumin inhibited the proliferation of U-251 cells and induced apoptosis of U-251 cells.level of ATPase in U-251 plasma Membrane was low remarkably.Conclusion:Curcumin induced apoptosis and inhibited proliferation of U-251 cell via inhibition of activation of plasma Membrane ATPase.

关键词

姜黄素胶质瘤细胞U-251ATP酶凋亡

Keywords

curcuminHuman glioma cell U-251ATPaseapoptosis

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