Genetic polymorphism of 15 STR loci in Chongqing Tujia population

DENG Shi-xiong; et al

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Genetic polymorphism of 15 STR loci in Chongqing Tujia population

更新时间：2024-01-17

- Genetic polymorphism of 15 STR loci in Chongqing Tujia population
- Journal of Chongqing Medical University Vol. 34, Issue 11, Pages: 1499-1502(2009)
- 作者机构：
  
  重庆医科大学基础医学院法医学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394;D919.4
- Published：2009，
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[1]邓世雄,陈伟.重庆土家族群体15个STR基因座的遗传多态性[J].重庆医科大学学报,2009,34(11):1499-1502.

DENG Shi-xiong, et al. Genetic polymorphism of 15 STR loci in Chongqing Tujia population. [J]. Journal of Chongqing Medical University 34(11):1499-1502(2009)
[1]邓世雄,陈伟.重庆土家族群体15个STR基因座的遗传多态性[J].重庆医科大学学报,2009,34(11):1499-1502. DOI：

DENG Shi-xiong, et al. Genetic polymorphism of 15 STR loci in Chongqing Tujia population. [J]. Journal of Chongqing Medical University 34(11):1499-1502(2009) DOI：

摘要

目的:研究重庆土家族人群无关个体的15个STR基因座（CSF1PO、D13S317、D16S539、D18S51、D19S433、D21S11、D2S1338、D3S1358、D5S818、D7S820、D8S1179、FGA、TH01、TPOX、vWA）的遗传多态性。方法:应用AmpFlSTR Identifiler荧光标记复合扩增系统对115名重庆土家族无关个体血样DNA进行15个STR基因座的复合扩增

用ABI310遗传分析仪对扩增产物进行检测

用GeneScan、GenoTyper软件进行基因分型

统计计算15个STR基因座的群体遗传学参数。结果:15个STR基因座均符合Hardy-Weinberg平衡

杂合度在0.634 8～0.887 0之间

累积匹配概率和累积非父排除率分别为3.12×10-17和0.999 997 688 3。结论:15个STR基因座的累积匹配概率和累积非父排除率较高

适用于法医学亲子鉴定和个人识别。

Abstract

Objective:To investigate the genetic polymorphism of15 short tandem repeat(STR)loci(CSF1PO

D13S317

D16S539

D18S51

D19S433

D21S11

D2S1338

D3S1358

D5S818

D7S820

D8S1179

FGA

TH01

TPOXand vWA)in ChongqingTujia population.Methods:DNA samples from 115 Tujia unrelated individuals in Chongqing were screened with the use of AmpFlSTR Identifiler PCR Amplification Kit and ABI310 Analyzer.The relative fragment’s lengths of PCR products were calculated by gene scan analysis software and afterward genotyped by genotype software.Results:All the 15 loci met Hardy-Weinberg equilibrium.The heterozygosity(H)were ranged from 0.6348 to 0.8870.The combined probability of matching(Pm)and power of exclusion(PE)were determined to be 3.12× 10-17 and 0.999 997 688 3.Conclusion:A higher measured value could be obtained for both the combined probability of matching and power of exclusion:The 15 STR loci can meet the needs of the paternity testing and individual identification in forensic medicine.

关键词

土家族短串联重复序列遗传多态性法医学

Keywords

Tujia nationalitySTRgenetic polymorphismforensic medicine

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