Study of insulin resistance and microinflammation in the adipocytes of type 2 diabetics

HUANG Chao-li; et al

您当前的位置：

首页 >

文章列表页 >

Study of insulin resistance and microinflammation in the adipocytes of type 2 diabetics

更新时间：2024-01-17

- Study of insulin resistance and microinflammation in the adipocytes of type 2 diabetics
- Journal of Chongqing Medical University Vol. 34, Issue 11, Pages: 1502-1505(2009)
- 作者机构：
  
  1. 四川成都铁路中心医院肾内科
  2. 重庆医科大学附属第二医院肾内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R587.1
- Published：2009，
扫描看全文
[1]黄朝莉,钟玲,陈建伟.2型糖尿病患者脂肪细胞胰岛素抵抗与微炎症的研究[J].重庆医科大学学报,2009,34(11):1502-1505.

HUANG Chao-li, et al. Study of insulin resistance and microinflammation in the adipocytes of type 2 diabetics. [J]. Journal of Chongqing Medical University 34(11):1502-1505(2009)
[1]黄朝莉,钟玲,陈建伟.2型糖尿病患者脂肪细胞胰岛素抵抗与微炎症的研究[J].重庆医科大学学报,2009,34(11):1502-1505. DOI：

HUANG Chao-li, et al. Study of insulin resistance and microinflammation in the adipocytes of type 2 diabetics. [J]. Journal of Chongqing Medical University 34(11):1502-1505(2009) DOI：

摘要

目的:观察2型糖尿病患者脂肪细胞内胰岛素信号转导蛋白:胰岛素受体底物-1（Insulin receptor substrate-1

IRS-1）酪氨酸磷酸化及蛋白激酶B（Protein kinase B

PKB/AKT）Ser473磷酸化水平以及微炎症因子白细胞介素6（Interleu kin-6

IL-6）的表达。方法:取2型糖尿病患者（2型糖尿病组）及非糖尿病患者（非糖尿病对照组）腹部皮下脂肪组织原代培养

用免疫沉淀法及蛋白印迹法（Western blot）检测两组脂肪细胞内胰岛素刺激后IRS-1酪氨酸磷酸化及AKT-Ser473磷酸化程度

酶联免疫吸附法检测脂肪细胞培养液中IL-6蛋白的水平。结果:（1）2型糖尿病组脂肪细胞内胰岛素刺激后IRS-1酪氨酸磷酸化及AKT-Ser473磷酸化水平明显低于非糖尿病对照组

差异有统计学意义（P<0.05）;（2）2型糖尿病组脂肪细胞培养液中IL-6蛋白的水平明显高于非糖尿病对照组

差异有统计学意义（P<0.05）。结论:2型糖尿病患者脂肪细胞存在胰岛素抵抗和微炎症状态。

Abstract

Objective:to investigate the insulin signal transduction protein in the adipocytes of Type 2 diabetics:Insulin Receptor Substrate-1(IRS-1)tyrosine phosphorylation and Protein Kinase B(PKB/AKT) Ser473 phosphorylation level and expression of microinflammatory cytokine interleukin-6(IL-6).Methods:Usingprimary cell culture

adipocytes from abdominal subcutaneous adipose tissue of Type 2 diabetics(Type 2 diabetes group) and non-diabetics(non-diabetes control group)were cultured.IRS-1 tyrosine phosphorylation and AKT-Ser473 phosphorylation level in the adipocytes of the two groups with insulin stimulation was detected by the Immunoprecipitation and western blot.IL-6 level in adipocytes culture supernatant was evaluated by ELISA.Results:(1) The IRS-1 tyrosine phosphorylation and AKT-Ser473 phosphorylation level in the adipocytes with insulin stimulation of Type 2 diabetes group were significantly lower than that of the non-diabetes control group(P<0.05);(2)The IL-6 protein level in the adipocytes medium of Type 2 diabetes group was significantly higher than that of the non-diabetes control group(P<0.05).Conclusion:The adipocytes of Type 2 diabetics present insulin resistance and microinflammatory states.

关键词

2型糖尿病脂肪细胞胰岛素抵抗微炎症

Keywords

Diabetestype2adipocytesinsulin resistanceMicroinflammation

references

Views

332

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

The role of RNA m⁶A methylation in insulin resistance in adipocytes

Mediating role of TYG-BMI in vitamin D and nonalcoholic fatty liver disease

Association between glucose metabolism disorder and prevalence of thyroid nodule in the elderly in iodine-adequate area

Association of serum irisin and insulin resistant in female with polycystic ovary syndrome

Association between body mass index and the incidence of type 2 diabetes mellitus

Related Author

Ye Diwen

Zhang Bingyang

Zhang Dantong

Ma Wanshan

Lu Sumei

Sun Wenwen

Xing Yuling

Huo Lijing

Related Institution

Department of Laboratory Medicine，The First Affiliated Hospital of Shandong First Medical University/Shandong Provincial Qianfoshan Hospital，Shandong Provincial Medicine and Health Key Laboratory of Clinical Laboratory Diagnostics

Cheeloo College of Medicine，Shandong University

School of Medical Laboratory，Weifang Medical University

Graduate School，North China University of Science and Technology

Department of Endocrinology， Hebei General Hospital

⁰