The effects of G<sub>0</sub>S<sub>2</sub> on the differentiation of mouse mallow stroma cells into adipocytes

LIN Xian-he; et al

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The effects of G₀S₂ on the differentiation of mouse mallow stroma cells into adipocytes

更新时间：2024-01-17

- The effects of G₀S₂ on the differentiation of mouse mallow stroma cells into adipocytes
- Journal of Chongqing Medical University Vol. 34, Issue 11, Pages: 1495-1498(2009)
- 作者机构：
  
  1. 安徽医科大学第一附属医院心内科
  2. 解放军306医院心内科
  3. 第三军医大学新桥医院心内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.23
- Published：2009，
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[1]林先和,彭佑华,李隆贵.G_0S_2基因参与调节鼠骨髓基质细胞向脂肪细胞分化[J].重庆医科大学学报,2009,34(11):1495-1498.

LIN Xian-he, et al. The effects of G₀S₂ on the differentiation of mouse mallow stroma cells into adipocytes. [J]. Journal of Chongqing Medical University 34(11):1495-1498(2009)
[1]林先和,彭佑华,李隆贵.G_0S_2基因参与调节鼠骨髓基质细胞向脂肪细胞分化[J].重庆医科大学学报,2009,34(11):1495-1498. DOI：

LIN Xian-he, et al. The effects of G₀S₂ on the differentiation of mouse mallow stroma cells into adipocytes. [J]. Journal of Chongqing Medical University 34(11):1495-1498(2009) DOI：

摘要

目的:探讨G0S2在调节鼠骨髓基质细胞向脂肪细胞分化过程中的作用。方法:利用原代培养小鼠骨髓基质细胞（Marrow strom cell

MSC）

脂质体转染法将表达质粒pCAG-PPARγ2及含报告基因的pGL2-COL1A质粒共转染至MSC

G418筛选

RT-PCR检测PPARγ及G0S2 mRNA表达

免疫组化检测PPARγ的表达

westen blot检测G0S2的表达

油红O染色检测细胞内脂滴。结果:MSC在未分化状态下不表达G0S2

经PPARγ基因转染后

G0S2开始表达

转染细胞最终分化为脂肪细胞。结论:PPARγ通过调节G0S2启动子调节后者表达

进而调节MSC向脂肪细胞分化

G0S2基因参与调节MSC向脂肪细胞分化。

Abstract

Objective:To explore the effects of G0S2 in the differentiation of mouse mallow stroma cells(MSCs)into adipocytes.Methods:The primary MSCs were cultured.The eukaryotic expression plasmid vector pCAG-PPARγ2 and pGL2-COL1A reporter vector were cotransfected into MSCs with lipotransfection method followed by G418 selection.The expression levels of PPARγmRNA and G0S2 mRNA were detected by RT-PCR.Immunohistochemistry and Westen blot were emplored to study the protein expression of PPARγ and G0S2.Lipid droplets in the cells were stained with Oil Red O.Results:No expression of G0S2 mRNA was found in undifferentiated MSCs.The G0S2 mRNA expressed in the MSCs after transfected with pCAG-PPARγ2

and the cells finaly differentiated into adipocytes.Conclusion:PPARγ stimulated G0S2 promoter activity via the PPRE in vivo.Then the expressions of G0S2 can regulate the adipocyte differentiaiton of MSCs.

关键词

骨髓基质细胞PPARγG0S2

Keywords

Mallow stroma cellsPPARγG0S2

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The effects of G0S2 on the differentiation of mouse mallow stroma cells into adipocytes

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摘要

Abstract

关键词

Keywords

references

The effects of G₀S₂ on the differentiation of mouse mallow stroma cells into adipocytes