Research of UVA in affecting Cx43 protein among rabbit lens epithelia cells

PENG Yan-li; et al

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Research of UVA in affecting Cx43 protein among rabbit lens epithelia cells

更新时间：2024-01-17

- Research of UVA in affecting Cx43 protein among rabbit lens epithelia cells
- Journal of Chongqing Medical University Vol. 34, Issue 7, Pages: 899-902(2009)
- 作者机构：
  
  重庆医科大学附属第二医院眼屈光矫治中心
- 作者简介：
- 基金信息：
- DOI：
  CLC： R776.1
- Published：2009，
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[1]彭艳丽,李立,汤永强.近紫外线对兔晶状体上皮细胞Cx43蛋白影响的研究[J].重庆医科大学学报,2009,34(07):899-902.

PENG Yan-li, et al. Research of UVA in affecting Cx43 protein among rabbit lens epithelia cells. [J]. Journal of Chongqing Medical University 34(7):899-902(2009)
[1]彭艳丽,李立,汤永强.近紫外线对兔晶状体上皮细胞Cx43蛋白影响的研究[J].重庆医科大学学报,2009,34(07):899-902. DOI：

PENG Yan-li, et al. Research of UVA in affecting Cx43 protein among rabbit lens epithelia cells. [J]. Journal of Chongqing Medical University 34(7):899-902(2009) DOI：

摘要

目的:探讨近紫外线抑制兔晶状体上皮细胞通讯功能的机制。方法:将兔晶状体前囊膜行组织块细胞培养

2～3代细胞分为正常对照组和近紫外线照射组（同一近紫外线光源下一固定位置50cm

测得该点的功率为0.2mW/cm2

给于同一强度

5、10、15min不同时间的近紫外线照射）

应用Western-blot方法

从翻译水平检测近紫外线对兔晶状体上皮细胞（Rabbit lens epithelia cells

RLECs）的连接蛋白43（Connexin43

Cx43）表达变化的影响;采用间接免疫荧光染色法

对RLECs的Cx43蛋白进行定位分析。结果:Cx43蛋白在各组晶状体上皮细胞中均有表达

并且正常对照组为高表达

随着近紫外线照射时间的增加

其Cx43蛋白的表达逐渐降低;并经近紫外线照射后正常定位于晶状体上皮细胞膜上的Cx43发生了内移。结论:证实近紫外线抑制晶状体上皮细胞通讯功能的机制可能与细胞缝隙连接通讯（Gap junction-mediated intercellular communication

GJIC）的关键蛋白Cx43蛋白表达的下降和功能定位的改变有关。

Abstract

Objective:To probe the mechanism of inhibition in gap junction-mediated intercellular communication (GJIC) of rabbit lens epithelia cells(RLECs) irradiated by UVA. Methods:RLECs of 2～3 generation were divided into normal control group and ultraviolet irradiation group (The UV intensity was 0.2 mW/cm2 under a UV lamp with fixed position 50 cm

RLECs were exposed to ultraviolet lamp at different times: 5

15 minutes). Western blot techniques were employed to detect the protein levels of Cx43 irradiated by UVA. By indirect immunofluorescence histochemistry we analyzed the localization of Cx43 in the RLECs. Results:In UVA irradiation group and normal control group

there was expression of Cx43. With the increasing intensity

the expression of Cx43 was decreasing. The immunnostaining of Cx43 in RLECs revealed that the internalization of Cx43 from membrance to plasma and nuclear was found in UVA irradiated RLECs. Conclusion: Cell molecule level study proved UVA irradiation can reduce GJIC of RLECs

which wis associated with perturbations in localization and number of Cx43 among RLECs.

关键词

近紫外线晶状体上皮细胞缝隙连接细胞通讯Cx43蛋白蛋白印迹法

Keywords

Ultraviolet A(UVA)Lens epithelia cellGap junction-mediated intercellular communicationConnexins43Western Blot

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