Protective effect of PI3K/Akt signaling pathway in puerarin pretreatment against myocardial ischemia-reperfusion injury in rat

MA Ya-fei; et al

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Protective effect of PI3K/Akt signaling pathway in puerarin pretreatment against myocardial ischemia-reperfusion injury in rat

更新时间：2024-01-17

- Protective effect of PI3K/Akt signaling pathway in puerarin pretreatment against myocardial ischemia-reperfusion injury in rat
- Journal of Chongqing Medical University Vol. 34, Issue 12, Pages: 1673-1676(2009)
- 作者机构：
  
  重庆医科大学附属第一医院麻醉科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5
- Published：2009，
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[1]马亚飞,刘新伟,文婷婷,乔欣.葛根素预处理减轻大鼠心肌缺血再灌注损伤及PI3K/AKT信号通路的作用[J].重庆医科大学学报,2009,34(12):1673-1676.

MA Ya-fei, et al. Protective effect of PI3K/Akt signaling pathway in puerarin pretreatment against myocardial ischemia-reperfusion injury in rat. [J]. Journal of Chongqing Medical University 34(12):1673-1676(2009)
[1]马亚飞,刘新伟,文婷婷,乔欣.葛根素预处理减轻大鼠心肌缺血再灌注损伤及PI3K/AKT信号通路的作用[J].重庆医科大学学报,2009,34(12):1673-1676. DOI：

MA Ya-fei, et al. Protective effect of PI3K/Akt signaling pathway in puerarin pretreatment against myocardial ischemia-reperfusion injury in rat. [J]. Journal of Chongqing Medical University 34(12):1673-1676(2009) DOI：

摘要

目的:观察葛根素（Puerarin

Pue）预处理对缺血再灌注大鼠心肌的保护作用及其可能机制。方法:40只SD大鼠随机分为5组（n=8）:假手术组（SH组）;缺血再灌注组（I/R组）;葛根素预处理组（PU组）;葛根素预处理+LY294002组（PU+LY组）;缺血再灌注+LY294002组（I/R+LY组）。结扎冠状动脉前降支30min

再灌注120min。于缺血前、缺血30min以及再灌注60、90、120min时

测定左室收缩压（Lef tventricular systolic pressure

LVSP）、左心室舒张末压（Left ventricular end-diastolic pressure

LVEDP）、左室内压变化最大上升和下降速率（Maximum rate of left ventricular pressure development and decay

±dp/dtmax）;测定心肌梗死面积百分比、一氧化氮（Nitric oxide

NO）和一氧化氮合酶（Nitric-oxide synthase

NOS）的含量。结果:I/R组较SH组各时间点的收缩及舒张功能显著降低

心肌梗死面积增加

NO和NOS含量明显降低;与I/R组比较

PU组各时间点的收缩及舒张功能下降程度明显减轻

心肌梗死面积明显减少

NO、NOS含量明显增加;PU+LY组与PU组相比各时点的收缩及舒张功能显著降低

心肌梗死面积增加

NO和NOS含量明显降低;PU+LY组、I/R+LY组与I/R组相比以上指标差异均无显著意义。结论:葛根素预处理对缺血再灌注大鼠心肌有较好的保护作用

其作用机制可能与PI3K/Akt信号通路的活化有关。

Abstract

Objective:To investigate the protective effect of preconditioning with Puerarin on myocardial ischemia reperfusion in adult SD rats and its possible mechanism.Methods:Forty Male SD rats were divided randomly into five groups(n=8).The myocardial injury of rats ware produced by the ligation of left coronary artery for 30 min followed by 120 min reperfusion.LVSP

LVEDP

and ±dp/dtmax were recorded at ischemia 30 min

reperfusion 60

90 and 120 min.The myocardial infarct size and the level of NO and NOS were observed.Results:In group I/R

the systolic and diastolic function were significantly reduced;myocardial infarct size was increased;NO and NOS were significantly lowered compared with those in group SH(P<0.05).The systolic and diastolic function were improved;myocardial infarct size was significantly smaller;the level of NO and NOS were increased significantly in group PU as compared with those in group I/R(P<0.05).All these parameters in group PU+LY were not improved compared with those in group PU(P<0.05).There was no significant difference among the parameters of groups PU+LY

I/R+LY

and I/R.Conclusion:Preconditioning with puerarin can significantly reduce myocardial ischemia-reperfusion injury

the mechanism of which is partly via PI3K-Akt signal transduction.

关键词

葛根素心肌缺血再灌注磷脂酰肌醇-3激酶丝(苏)氨酸激酶

Keywords

PuerarinmyocardiumIschemia reperfusionPI3KAKT

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