Effect of HIFU combined with chemotherapy on apoptosis and proliferation of glioma cell in nude mice

XIAO Gang; et al

您当前的位置：

首页 >

文章列表页 >

Effect of HIFU combined with chemotherapy on apoptosis and proliferation of glioma cell in nude mice

更新时间：2024-01-17

- Effect of HIFU combined with chemotherapy on apoptosis and proliferation of glioma cell in nude mice
- Journal of Chongqing Medical University Vol. 34, Issue 11, Pages: 1475-1478(2009)
- 作者机构：
  
  1. 重庆市第一人民医院神外科
  2. 重庆医科大学附属第一医院神经外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.41
- Published：2009，
扫描看全文
[1]肖刚,霍钢.HIFU联合化疗对裸鼠皮下胶质瘤细胞凋亡及增殖的影响研究[J].重庆医科大学学报,2009,34(11):1475-1478.

XIAO Gang, et al. Effect of HIFU combined with chemotherapy on apoptosis and proliferation of glioma cell in nude mice. [J]. Journal of Chongqing Medical University 34(11):1475-1478(2009)
[1]肖刚,霍钢.HIFU联合化疗对裸鼠皮下胶质瘤细胞凋亡及增殖的影响研究[J].重庆医科大学学报,2009,34(11):1475-1478. DOI：

XIAO Gang, et al. Effect of HIFU combined with chemotherapy on apoptosis and proliferation of glioma cell in nude mice. [J]. Journal of Chongqing Medical University 34(11):1475-1478(2009) DOI：

摘要

目的:探讨高强度聚焦超声（High intensity focused ultrosound

HIFU）联合化疗对裸鼠皮下胶质瘤靶区周围细胞凋亡和增殖的影响。方法:建立人（U251）胶质瘤移植模型

随机分入3组

局部分别行单HIFU治疗、单盐酸尼莫司汀（Nimustine

ACNU）及HIFU联合ACNU治疗。治疗24 h后切取肿瘤组织

行HE染色观察组织病理变化

免疫组织化学法检测增殖细胞核抗原（Proliferating cell nuclear antigen

PCNA）的表达和TUNEL法检测细胞凋亡。治疗7 d后分别测量3组瘤块体积。结果:HIFU联合化疗组靶区周围组织凋亡细胞数较单HIFU组及单化疗组高（P<0.01）;而PCNA阳性细胞数较后两者降低（P<0.01）。联合治疗组肿瘤体积较单HIFU组及单化疗组缩小（P<0.01）。结论:HIFU治疗及化疗在胶质瘤的治疗中具有协同作用。HIFU联合化疗能明显提高HIFU治疗疗效

诱导靶区周围胶质瘤细胞凋亡并抑制其增殖发挥抗肿瘤作用

有利于胶质瘤的治疗。

Abstract

Objective:To explore the changes of cellapoptosis and proliferation in the periphery of the target area on glioma subcutaneous transplanted model in nude mice treated with high inensity focused ultrasound(HIFU)in combination with chemotherapy.Methods: Experimental nude mice of subcutaneous transplanted models with human glioma(U251)was established.These nude mice bearing subcutaneous human neuron-gliocytoma xenografts were divided into three groups randomly

HIFU in combination with ACNU(Nimustine) adjuvant themotherapy group

single chemotherapy group and single HIFU group.24 hours after tumors were treated

the pathological characteristics

apoptosis and proliferating cell nuclear antigen(PCNA)in tumor tissues were tested by HE staining examination

TdT-me diated UTP nick end labeling(TUNEL)and immunohistochemical staining respectively.7 days later

the volume of the xenografts was measured.Results:After HIFU ablation

in the periphery of the target area

the percentage of PCNA in the HIFU in combination with adjuvant chemotherapy group was significantly less than that in single chemotherapy group and single HIFU group(P<0.01)and the apoptosis cells were more than these in the last two groups(P<0.01).The volume oftumor tissues in HIFU in combination with ACNU group was smaller than that in single chemotherapy group and single HIFU group(P<0.01).Conclusion:There were marked synergstic effects of both HIFU ablation and chemotherapy on U251 glioma in nude mice.In the periphery of the target area

HIFU in combination with adjuvant chemotherapy could enhance the HIFU therapeutic effects obviously

and also induce U251cell apoptosis and restrain its proliferation in glioma

thus it would promote the therapeutic efficacyfor glioma.

关键词

神经胶质瘤高强度聚焦超声细胞凋亡增殖细胞核抗原化疗

Keywords

gliomaHigh intensive focused ultrasoundapoptosisproliferating cell nuclear antigenchemotherapy

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of Glycyrrhiza uralensis Fisch. endophytes combined with cisplatin on proliferation and apoptosis of A549 cells

Milk fat globule-EGF factor Ⅷ attenuates apoptosis and permeability damage of the intestinal mucosa in rats with obstructive jaundice

Comparison of the clinical efficacy of Disitamab Vedotin and postoperative chemotherapy in the treatment of HER-2 positive locally advanced urothelial carcinoma

Effect of andrographolide on TBHP-induced apoptosis of intervertebral disc nucleus pulposus cells and its mechanism

Effect of progressive muscle relaxation training on coping style and emotion of patients with lung cancer

Related Author

Zhang Shanglong

Zhang Nan

Lian Xiaolong

Ye Liqiao

Ma Quhuan

Deng Yi

Guo Yong

Tang Yongliang

Related Institution

College of Pharmacy，Gansu University of Chinese Medicine

Department of Hepatobiliary Pancreatic Surgery,Daping Hospital,Army Medical University

Department of Hepatobiliary Pancreatic Surgery,People’s Hospital of Chongqing Liang Jiang New Area

Department of Urology，The First Affiliated Hospital of Chongqing Medical University

Department of Orthopedics，Wuhan Third Hospital

⁰