Effect of liposome adriamycin on proliferation and apoptosis of HNE-1 cell line

DENG Bi; et al

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Effect of liposome adriamycin on proliferation and apoptosis of HNE-1 cell line

更新时间：2024-01-17

- Effect of liposome adriamycin on proliferation and apoptosis of HNE-1 cell line
- Journal of Chongqing Medical University Vol. 34, Issue 11, Pages: 1479-1481(2009)
- 作者机构：
  
  1. 重庆医科大学附属第一医院耳鼻咽喉科
  2. 重庆医科大学附属第一医院胸心外科
  3. 重庆医科大学药学院
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.63
- Published：2009，
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[1]邓碧,叶琳,王驰,陈鸿雁.脂质体阿霉素对人鼻咽癌HNE-1细胞株增殖与凋亡作用的研究[J].重庆医科大学学报,2009,34(11):1479-1481.

DENG Bi, et al. Effect of liposome adriamycin on proliferation and apoptosis of HNE-1 cell line. [J]. Journal of Chongqing Medical University 34(11):1479-1481(2009)
[1]邓碧,叶琳,王驰,陈鸿雁.脂质体阿霉素对人鼻咽癌HNE-1细胞株增殖与凋亡作用的研究[J].重庆医科大学学报,2009,34(11):1479-1481. DOI：

DENG Bi, et al. Effect of liposome adriamycin on proliferation and apoptosis of HNE-1 cell line. [J]. Journal of Chongqing Medical University 34(11):1479-1481(2009) DOI：

摘要

目的:研究脂质体阿霉素对人鼻咽癌HNE-1细胞株增殖抑制和凋亡作用的影响。方法:本研究以人鼻咽癌HNE-1细胞株为研究对象

以游离阿霉素为对照

用四甲基偶氮唑蓝（MTT）法测定脂质体阿霉素对HNE-1的细胞毒作用;分别采用倒置显微镜和TUNEL法检测脂质体阿霉素作用于HNE-1细胞后细胞凋亡的形态学变化和细胞凋亡率。结果:脂质体阿霉素对HNE-1细胞的IC50是游离阿霉素的1/3倍;与游离阿霉素组相比较

倒置显微镜观察脂质体阿霉素组

发现该实验组细胞出现细胞凋亡形态学变化;脂质体阿霉素和游离阿霉素分别作用于HNE-1细胞的凋亡指数分别为11.27%±0.33%、43.57%±3.14%。结论:本实验研究显示

与游离阿霉素相比较

脂质体阿霉素对人鼻咽癌HNE-1细胞株具有较好的体外增殖抑制作用

能够诱导HNE-1细胞凋亡。

Abstract

Objective:To compare the effects of liposome adriamycin and free adriamycin on the cell proliferation and apoptosis of HNE-1 cell line.Methods:MTT reduction assay was used to investigate the effects of liposome adriamycin and free adriamycinon on the proliferation of HNE-1 cell line.Inverted microscope was used to observe the cell morphology variation.Flowcytometry(FCM)was used to detecet the apoptosis rate of HNE-1 cell.Results:The IC50 of HNE-1 cell line by free adriamycin was 300% compared to that by liposome adriamycin.After treated with liposome adriamycin

typical apoptosis morphological character appeared on HNE-1 cell line.After treated with liposome adriamycin and free adriamycin respectively

the apoptosis rates of HNE-1 were:11.27%±0.33%and 43.57%± 3.14%respectively.Conclusion:The rate of the cell proliferation by liposome adriamycin was larger than that by free adriamycin

and the rate of the HNE-1 cell line apoptosis by liposome adriamycin on was larger than that by free adriamycin.

关键词

脂质体阿霉素HNE-1细胞凋亡

Keywords

liposomesAdriamycinHNE-1apoptosis

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