Influence of mycophenolic acid on survival prolongation of renal allografts with tolerogenic dendritic cells

LI Feng; et al

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Influence of mycophenolic acid on survival prolongation of renal allografts with tolerogenic dendritic cells

更新时间：2024-01-17

- Influence of mycophenolic acid on survival prolongation of renal allografts with tolerogenic dendritic cells
- Journal of Chongqing Medical University Vol. 34, Issue 9, Pages: 1141-1143(2009)
- 作者机构：
  
  1. 重庆市三峡中心医院泌尿外科
  2. 第三军医大学新桥医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392.1
- Published：2009，
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[1]李风,冯嘉瑜,肖亚,王平贤,范明齐,张银甫,黄赤兵.霉酚酸合并致耐受树突状细胞延长小鼠心脏移植物的研究[J].重庆医科大学学报,2009,34(09):1141-1143.

LI Feng, et al. Influence of mycophenolic acid on survival prolongation of renal allografts with tolerogenic dendritic cells. [J]. Journal of Chongqing Medical University 34(9):1141-1143(2009)
[1]李风,冯嘉瑜,肖亚,王平贤,范明齐,张银甫,黄赤兵.霉酚酸合并致耐受树突状细胞延长小鼠心脏移植物的研究[J].重庆医科大学学报,2009,34(09):1141-1143. DOI：

LI Feng, et al. Influence of mycophenolic acid on survival prolongation of renal allografts with tolerogenic dendritic cells. [J]. Journal of Chongqing Medical University 34(9):1141-1143(2009) DOI：

摘要

目的:探讨霉酚酸（Mycophenolic acid

MPA）对致耐受性树突状细胞（Dendriti ccells

DC）延长小鼠异位移植心存活效应的影响。方法:以Balb/C、C57小鼠为心脏移植供、受体

在供体DC培养过程中加入3种浓度MPA

以CTLA-4Ig基因转染DC使之成为表达CTLA-4Ig的致耐受DC;观察DC形态变化并检测受体脾细胞对上述DC的刺激反应。将未处理的致耐受DC以及0.05μmol/LMPA处理DC于心脏移植前72h分别注入受体（组3、组4）

注入供体DC的受体为组2

未注入DC的受体为对照（组1）

观察受体颈部移植心脏搏动时间以及心电图检查结果。结果:MPA组DC形态老化延缓

脾细胞对3种浓度MPA处理的DC刺激反应均显著低于对未经MPA处理致耐受DC的刺激反应;与对照相比

组1、组2移植心存活时间显著延长

差异有统计学意义（P<0.01）。结论:MPA预处理可降低致耐受DC的抗原提呈能力

提高其延长移植心存活的效能。

Abstract

Objective:To study the influence of mycophenolic acid(MPA) on survival prolongation of heart allografts with tolerogenic dendritic cells(DCs ) in rat s. Methods:The influence of MPA on CTLA-4Ig-gene transferred donor DCs was observed in vitro morphologically and functionally. The influence of MPA on the survival time of the heart allografts was observed in a Blab/C→C57 Cervical Heterotopic Heart transplantation model with recipients treated by CTLA-4Ig-gene transferred donor DCs. Results:The maturation and aging of DC were delayed and the stimulating ability of DC to recipient splenic cells was impaired significantly with the adding of MPA . Survival time of renal allograft s in CTLA-4Ig-gene transferred DC group was prolonged significantly and further prolonged with the adding of MPA. The allografts functioned well even after 45 d postoperatively in recipient t reated with MPA preconditioned DC. Conclusion:MPA preconditioning impairs the ability of antigen presentation of CTLA-4Ig-gene transferred donor DCs and enhances it s efficacy of survival prolongation of heart allografts in mice.

关键词

霉酚酸树突状细胞心脏移植

Keywords

Mycophenolic aciddendritic cellsHeart transplantation

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