Bioinformatics analysis of expressed sequence tag associated with stem cells in tongue squamous carcinoma

ZOU Bo; JI Ping; SUN Shou-juan; QI Xiao-juan

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Bioinformatics analysis of expressed sequence tag associated with stem cells in tongue squamous carcinoma

更新时间：2024-01-17

- Bioinformatics analysis of expressed sequence tag associated with stem cells in tongue squamous carcinoma
- Journal of Chongqing Medical University Vol. 36, Issue 10, Pages: 1233-1236(2011)
- 作者机构：
  
  重庆医科大学附属口腔医院颌面外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R739.86
- Published：2011，
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[1]邹波,季平,孙守娟,漆小娟.舌鳞癌干细胞相关差异表达序列标签的生物信息学分析[J].重庆医科大学学报,2011,36(10):1233-1236.

ZOU Bo, JI Ping, SUN Shou-juan, et al. Bioinformatics analysis of expressed sequence tag associated with stem cells in tongue squamous carcinoma. [J]. Journal of Chongqing Medical University 36(10):1233-1236(2011)
[1]邹波,季平,孙守娟,漆小娟.舌鳞癌干细胞相关差异表达序列标签的生物信息学分析[J].重庆医科大学学报,2011,36(10):1233-1236. DOI：

ZOU Bo, JI Ping, SUN Shou-juan, et al. Bioinformatics analysis of expressed sequence tag associated with stem cells in tongue squamous carcinoma. [J]. Journal of Chongqing Medical University 36(10):1233-1236(2011) DOI：

摘要

目的:对利用抑制消减杂交技术获得的舌鳞癌干细胞相关表达序列标签（Expressed squence tag

EST）进行电子克隆及序列分析。方法:利用互联网上的公共数据库及生物信息学分析软件对获得的舌鳞癌干细胞相关差异表达序列标签进行核酸序列和蛋白质序列分析

探索克隆差异表达基因的研究方法和策略。结果:通过电子延伸得到1个1 705 bp的cDNA序列;核酸序列分析显示

该cDNA定位在1号染色体

含有1个编码146个氨基酸的完整开放阅读框;对氨基酸序列进行结构域预测显示其存在1个与Translin超家族同源的区域;BLAST比对显示该氨基酸序列与Translin相关蛋白X（Translin associatedprotein X

TRAX）具有较高的同源性。结论:利用生物信息学技术获得了舌鳞癌相关表达序列标签的基本信息

为深入研究该表达序列标签的功能奠定基础。

Abstract

Objective:To analyze the expressed squence tag(EST) associated with stem cells in tongue squamous carcinoma obtained by suppression subtractive hybrization(SSH).Methods:EST was acquired from stem cells in tongue squamous carcinoma by SSH.Bioinformatics analysis of stem cell related genes was done by the bioinformatic database and biosoftwares such as BLAST and ExPasy analyse soft box.Results:A 1 705 bp complete cDNA sequence was obtained by electronic clone.This cDNA sequence was sited in homo sapiens 1q42

and included a complete open reading frame(ORF) coding 146 amino acids.The analysis of its protein sequence indicated that this protein contained a structural domain similar to Translin superfamily.BLAST analysis revealed that this protein was homologous with Translin associated protein X(TRAX).Conclusion:Through bioinformatics analysis

we obtain the basic information about stem cell related EST in tongue squamous carcinoma

and this may provide potentially important insights into the function analysis of these ESTs.

关键词

肿瘤干细胞舌鳞癌表达序列标签生物信息学分析

Keywords

tumor stem celltongue squamous carcinomaexpressed sequence tagbioinformatics analysis

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