Objective:To construct human cytochrome enzyme P450 2A13(CYP2A13) and green fluorescent protein(GFP) co-expressing lentiviral vector

and transfect A549 lung cancer cells for highly expressing CYP2A13 gene continuously.Methods:Gene recombinant technology was employed to link CYP2A13 and GFP genes for constructing lentiviral vector.After transfecting 293T cells

virus was collected and the titer wasmeasured.The packed lentiviral vector was used to infect A549 cells.Then laser scanning confocal microscope(LSCM) were applied to detect the location of fusion protein GFP-CYP2A13

while real-time PCR and Western blot were applied to deteat and the expression of CYP2A13 in the infected A549 cells respectively.Results:A recombinant lentiviral vector co-expressing CYP2A13 and GFP gene was constructed successfully.After transfection

a large number of 293T cells with green fluorescence were observed under fluorescent microscope

and green fluorescence was seen in the nucleus and cytoplasm of A549 cells under LSCM.Real-time PCR and Western blot showed that CYP2A13 gene was highly expressed.Conclusion:Successful construction of human CYP2A13 and GFP co-expressing lentiviral vector provides a stable vector for further investigation of the relationship between CYP2A13 gene and respiratory tumors.