Objective:To explore catharsis effect and its efficiency matter in the mixture of leaf and leaf stalk in wasabi

and to provide basaes for development and utilization leaf and leaf stalk in wasabi that is by-product in wasabi planting as pharmaceutical application.Methods:The mixture of dried powder of wasabi leaf and leaf stalk was obtained by freeze-drying and crushing fresh leaves and leaf stalks.With enzymatic-gravimetric method

total dietary fibre

soluble dietary fibre(SDF) and insoluble dietary fibre(IDF) in mixture of dried powder are determined.Isothiocyanates in mixture of dried powder was removed by adding water and incubating in 37℃ overnight.The matters soluble in water were removed by adding water

incubating in 37℃ overnight

centrifugation in 5 000 r/min×10 min and precipitation.The effect were tested in moistening intestines and freeing the stool in mice(advancing movement of small intestine

the time of first black feces excretion

the numbers of feces in 6 h and the weight of feces in 6 h).The above samples included the mixture of dried powder of wasabi leaf and leaf stalk

that free of isothiocyanates and that free of water-soluble elements.Results:The total dietary fibre in mixture of dried powder of wasabi leaf and leaf stalk was 92.3 g/kg

soluble dietary fibre(SDF) was 48.2 g/kg

insoluble dietary fibre(IDF) was 44.1 g/kg and ratio of SDF and IDF is 1.1.As for the effect in moistening the intestines and freeing the stool

the above three samples(mixture of dried powder of wasabi leaf and leaf stalk

that free of isothiocyanates

and that free of water-soluble elements) had effect in enhancing small intestine movility

shortening the first time of cacation

increasing the amount of feces pellets in 6 h and total quantity of feces in 6 h.The comparison between the three samples in 3 groups showed significantly different(P<0.05).Conclusion:The total content of and soluble dietary fibre(SDF) in mixture of dried powder of wasabi leaf and leaf stalk is slightly high;it has effect in moistening the intestines and freeing stool for model animals by compound diphenoxylate

and the related function materials are isothiocyanates

water-soluble and-insoluble matters.