Comparative study of the biological characteristics of two human ovarian carcinoma models in nude mice

PU Cai-xiu; CHANG Shu-fang; SUN Jiang-chuan; LIU Hong-xia; ZHU Yi; CHEN Jia

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Comparative study of the biological characteristics of two human ovarian carcinoma models in nude mice

更新时间：2024-01-17

- Comparative study of the biological characteristics of two human ovarian carcinoma models in nude mice
- Journal of Chongqing Medical University Vol. 36, Issue 11, Pages: 1343-1346(2011)
- 作者机构：
  
  重庆医科大学附属第二医院妇产科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.31
- Published：2011，
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[1]蒲才秀,常淑芳,孙江川,刘红霞,朱轶,陈佳.2种人卵巢癌裸鼠移植瘤模型的生物学特性比较研究[J].重庆医科大学学报,2011,36(11):1343-1346.

PU Cai-xiu, CHANG Shu-fang, SUN Jiang-chuan, et al. Comparative study of the biological characteristics of two human ovarian carcinoma models in nude mice. [J]. Journal of Chongqing Medical University 36(11):1343-1346(2011)
[1]蒲才秀,常淑芳,孙江川,刘红霞,朱轶,陈佳.2种人卵巢癌裸鼠移植瘤模型的生物学特性比较研究[J].重庆医科大学学报,2011,36(11):1343-1346. DOI：

PU Cai-xiu, CHANG Shu-fang, SUN Jiang-chuan, et al. Comparative study of the biological characteristics of two human ovarian carcinoma models in nude mice. [J]. Journal of Chongqing Medical University 36(11):1343-1346(2011) DOI：

摘要

目的:建立人卵巢癌裸鼠腹腔及皮下移植瘤模型

比较2种模型的生物学特性。方法:采用细胞悬液注射法将人上皮性卵巢癌细胞A2780/DDP注射于裸鼠皮下或腹腔

建立人卵巢癌裸鼠移植瘤模型

观察模型动物肿瘤的生长规律及组织学特性

流式细胞术分析细胞周期分布

免疫组化检测促黄体激素释放激素受体（Luteinzing-hormone releasing hormone receptor

LHRH-R）表达情况。结果:裸鼠腹腔及皮下移植瘤模型的成瘤率均为100%

2组荷瘤鼠中位生存时间分别为（30.000±1.095）d和（41.000±0.894）d

2组移植瘤细胞周期分布与原代细胞周期分布相仿

且2种模型的组织形态学和超微结构相似

免疫组化显示均表达LHRH-R。结论:人卵巢癌细胞株A2780/DDP裸鼠皮下和腹腔移植瘤模型易于建立

腹腔移植瘤模型能更好地模拟人卵巢癌腹腔播散的生物学行为

是研究卵巢癌治疗较理想的动物模型。

Abstract

Objective:To establish intraperitoneal(IP) injection model and subcutaneous(SC) injection model of human ovarian carcinoma in nude mice and compare their biological characteristics.Methods:The cell suspension derived from A2780/DDP were injected intraperitoneally or subcutaneously in nude mice to establish human epithelial ovarian carcinoma models

and their growth behavior and biological characteristics were observed.Distribution of cell cycles were analyzed by flow cytometry(FCM).Expression of LHRH-R were investigated by immunohistochemistry.Results:The human ovarian carcinoma models were established successfully.The success rate of transplantation in both groups were 100%.The median survival time lengths in IP group and SC group were 30.000±1.095 days and 41.000±0.894 days

respectively.FCM analysis showed the distribution of cell cycles of xenografted tumors in two groups were the same as those of A2780/DDP cells.Histology and electron microscopy showed the same characteristics and morphological properties in the two groups

and immunohistochemistry staining of LHRH-R were both positive.Conclusion:Human ovarian carcinoma models in nude mice are easy to establish.And the IP model may be an ideal model for treatment research of ovarian carcinoma as it simulates the intraperitoneally dessiminating behavior of human ovarian carcinoma in the patients.

关键词

上皮性卵巢癌动物模型裸鼠促黄体激素释放激素

Keywords

epithelial ovarian carcinomaanimal modelnude miceluteinzing-hormone releasing hormone(LHRH)

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Related Institution

Pediatric Research Institute，the Children’s Hospital，Chongqing Medical University，Key Laboratory of Child Development and Disorders Founded by Ministry of Education

Department of Radiology，the Children’s Hospital，Chongqing Medical University

Department of Endocrinology，Chongqing Traditional Chinese Medicine Hospital

Department of Endocrinology，The Second Affiliated Hospital of Chongqing Medical University

Department of Dermatology,The First Affiliated Hospital of Chongqing Medical University

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