Reversal of multi-drug resistance by Zilongjin in human bladder cancer cell line

WANG Xiang-wei; ZHANG Yuan-ning; LI Jie; FANG Zhen-qiang; YI Shan-hong; YE Gang

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Reversal of multi-drug resistance by Zilongjin in human bladder cancer cell line

更新时间：2024-01-17

- Reversal of multi-drug resistance by Zilongjin in human bladder cancer cell line
- Journal of Chongqing Medical University Vol. 36, Issue 10, Pages: 1183-1185(2011)
- 作者机构：
  
  第三军医大学第二附属医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R273
- Published：2011，
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[1]王祥卫,张远宁,李杰,方针强,易善红,叶钢.紫龙金逆转人膀胱癌细胞多药耐药研究[J].重庆医科大学学报,2011,36(10):1183-1185.

WANG Xiang-wei, ZHANG Yuan-ning, LI Jie, et al. Reversal of multi-drug resistance by Zilongjin in human bladder cancer cell line. [J]. Journal of Chongqing Medical University 36(10):1183-1185(2011)
[1]王祥卫,张远宁,李杰,方针强,易善红,叶钢.紫龙金逆转人膀胱癌细胞多药耐药研究[J].重庆医科大学学报,2011,36(10):1183-1185. DOI：

WANG Xiang-wei, ZHANG Yuan-ning, LI Jie, et al. Reversal of multi-drug resistance by Zilongjin in human bladder cancer cell line. [J]. Journal of Chongqing Medical University 36(10):1183-1185(2011) DOI：

摘要

目的:研究中药紫龙金逆转人膀胱癌细胞多药耐药（Multidrug resistance

MDR）机理。方法:应用MTT法检测紫龙金的细胞杀伤作用

采用流式细胞仪检测细胞凋亡

RT-PCR法检测Bcl-2、Bax基因mRNA水平表达。结果:BIU-87/ADM细胞生存率为（98.00±1.48）%

加入紫龙金后（浓度分别为200、500μg/ml）

其细胞生存率分别为（68.14±5.62）%、（52.36±1.87）%

差异显著（P<0.01）;流式细胞仪检测发现:经200、500μg/ml的紫龙金处理后

BIU-87/ADM细胞凋亡率[（17.32±2.35）%、（22.76±3.65）%]明显高于对照组（5.86±1.37）%

差异显著（P<0.01）;RT-PCR结果显示

紫龙金可以下调Bcl-2基因的表达

可使Bax基因表达增强。结论:紫龙金可通过下调Bcl-2基因的表达

增强Bax基因表达诱导人膀胱癌多药耐药细胞凋亡。

Abstract

Objective:To study the reversal of multi-drug resistance by Zilongjin in human bladder cancer cell line.Methods:The cytotoxicity of Zilongjin on human bladder cancer cell line BIU-87/ADM was studied by MTT assay.Apoptosis was detected by flow cytometry;expression of Bcl-2/Bax gene was studied by RT-PCR.Results:BIU-87/ADM cell survival rate was(98.00±1.48)%.After being treated with Zilongjin

cell survival rate was(68.14±5.62)% and(52.36±1.87)%

respectively(P<0.01);apoptosis rate in cell line BIU-87 /ADM(treated with Zilongjin) was(17.32±2.35)% and(22.76±3.65)%

higher than that in control group cell line [(5.86±1.37)%

P<0.01].Bcl-2 gene expression was decreased

while Bax gene expression was increased according to RT-PCR assay.Conclusion:Apoptosis can be induced by Zilongjin through decreasing Bcl-2 and increasing Bax gene expressions in human bladder cancer multi-drug resistance cell line.

关键词

膀胱癌多药耐药性紫龙金细胞凋亡

Keywords

bladder canermultidrug resistanceZilongjinapoptosis

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