Inhibitory effect of cetuximab combined with paclitaxel on nasopharyngeal carcinoma cell line CNE-1 and its molecular mechanism

Liu Yang; Zhang Xingping; Yang Fan; Tang Long; Liu Mei

您当前的位置：

首页 >

文章列表页 >

Inhibitory effect of cetuximab combined with paclitaxel on nasopharyngeal carcinoma cell line CNE-1 and its molecular mechanism

更新时间：2023-12-18

- Inhibitory effect of cetuximab combined with paclitaxel on nasopharyngeal carcinoma cell line CNE-1 and its molecular mechanism
- Journal of Chongqing Medical University Vol. 38, Issue 5, Pages: 626-629(2014)
- 作者机构：
  
  重庆医科大学附属第一医院肿瘤科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2014，
扫描看全文
Liu Yang, Zhang Xingping, Yang Fan, et al. Inhibitory effect of cetuximab combined with paclitaxel on nasopharyngeal carcinoma cell line CNE-1 and its molecular mechanism. [J]. Journal of Chongqing Medical University 38(5):626-629(2014)
DOI：

Liu Yang, Zhang Xingping, Yang Fan, et al. Inhibitory effect of cetuximab combined with paclitaxel on nasopharyngeal carcinoma cell line CNE-1 and its molecular mechanism. [J]. Journal of Chongqing Medical University 38(5):626-629(2014) DOI：

摘要

目的：研究西妥昔单抗（Cetuximab）联合紫杉醇（Paclitaxel，PTX）对人鼻咽癌（nasopharyngeal carcinoma，NPC）CNE-1细胞增殖、凋亡的影响及其可能的分子机制。方法：培养人NPC CNE-1细胞株，随机分为空白对照组、Ｃetuximab组、ＰＴＸ组、联合用药组。用MTT法检测细胞抑制率，流式细胞仪检测细胞凋亡率，逆转录PCR（RT-PCR法）及Western blot法检测表皮生长因子受体（epidermal growth factor receptor，EGFR）、基质金属蛋白酶-2（matrix metalloproteinase-2，MMP-2）的mRNA和蛋白表达。结果：联合用药组的细胞抑制率高于Cetuximab组和PTX组（P值分别为0.002、0.000）；联合用药组的凋亡率高于Ｃetuximab组和ＰＴＸ组（P值均为0.000）；联合用药组的EGFR、MMP-2的mRNA表达均低于Ｃetuximab组和ＰＴＸ组（各组间EGFR mRNA的表达P值分别为0.000、0.001；MMP-2 mRNA的表达P值均为0.000）；联合用药组的EGFR、MMP-2的蛋白表达均低于Cetux－imab组和PTX组（各组间EGFR蛋白的表达P值均为0.000；MMP-2蛋白的表达P值分别为为0.000、0.006）。结论：Cetuximab联合PTX能够抑制人NPC CNE-1细胞的增殖，促进其凋亡，两者联用具有协同作用，其分子机制可能是通过抑制肿瘤细胞EGFR、MMP-2的表达来实现。

Abstract

Objective：To study the effects of cetuximab combined with paclitaxel on proliferation and apoptosis of nasopharyngeal car－cinoma cell line CNE-1 and its molecular mechanism. Methods：Nasopharyngeal carcinoma cell line CNE-1 were cultured and divid－ed into vacuity contrast group，cetuximab group，paclitaxel group and combination group. The inhibitory rate was detected by MTT，the apoptosis rate was analyzed by flow cytometry，and levels of epidermal growth factor receptor（EGFR），matrix metalloproteinase-2（MMP-2） were detected by RT-PCR and Western blot. Results：The inhibitory rate and apoptosis rate of combination group were higher than those of cetuximab group and paclitaxel group（P=0.002，P=0.000；P=0.000，P=0.000）. EGFR and MMP-2 mRNA levels of combination group were lower than those of cetuximab group and paclitaxel group（P=0.000，P=0.001；P=0.000，P=0.000）. EGFR and MMP-2 protein levels of combination group were lower than those of cetuximab group and paclitaxel group（P=0.000，P=0.000；P=0.000，P=0.006）. Conclusions：The combination of cetuximab and paclitaxel can increase the apoptosis and decrease the prolif－eration of nasopharyngeal carcinoma cell line CNE-1 with a synergistic action，the mechanism of which may through the inhibition of EGFR and MMP-2.

关键词

西妥昔单抗紫杉醇鼻咽癌增殖凋亡

Keywords

cetuximabpaclitaxelnasopharyngeal carcinomaproliferationapoptosis

references

Views

338

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Impacts of LncRNA MYLK-AS1 on proliferation，apoptosis，and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis

Knock-down RBX1 inhibits the proliferation，migration，and invasion of pancreatic cancer cells and promotes cell apoptosis by down-regulating PHF5A

Effect of GRASP65 expression on proliferation,apoptosis,migration and invasion of gastric cancer cells

Primary study on the mechanism of intralesional therapy for hemangioma with glucocorticoid

The effect of hepatocyte growth factor(HGF) on graft mucosal cell proliferation and apoptosis after rat small bowel transplantation

Related Author

Liu Jie

Xie Xingming

Niu Hongxia

Yang Xianzhi

Zhang Jing

Yan Youyou

Xu Dongchao

Zhang Bo

Related Institution

Department of Gastrointestinal Surgery，The Third Affiliated Hospital of Zunyi Medical University/ The First People’s Hospital of Zunyi

Translational Medicine Research Center, Affiliated Hangzhou First People’s Hospital，Zhejiang University School of Medicine

Department of Gastroenterology, Affiliated Hangzhou First People’s Hospital，Zhejiang University School of Medicine

Department of Pathology,Molecular Medicine and Cancer Research Cancer,Chongqing Medical University

Department of Gynaecology and Obstetrics,The First Affiliated Hospital of Chongqing Medical University

⁰