Promotion of bone morphogenetic protein 9-induced mesenchymal stem cell osteogenesis by transforming growth factor-?茁1

Li Ruidong; DENG Zhongliang; Wang Pan; Kong Yu; Wang Yang; Yin Liangjun; Chen Liang

您当前的位置：

首页 >

文章列表页 >

Promotion of bone morphogenetic protein 9-induced mesenchymal stem cell osteogenesis by transforming growth factor-?茁1

更新时间：2023-12-18

- Promotion of bone morphogenetic protein 9-induced mesenchymal stem cell osteogenesis by transforming growth factor-?茁1
- Journal of Chongqing Medical University Vol. 38, Issue 5, Pages: 621-625(2014)
- 作者机构：
  
  1. 重庆医科大学附属第二医院骨科,重庆,400010
  2. 重庆市急救医疗中心创伤外科,重庆,400014
  3. 重庆市传染病医疗救治中心急诊科,重庆,400036
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2014，
扫描看全文
Li Ruidong, DENG Zhongliang, Wang Pan, et al. Promotion of bone morphogenetic protein 9-induced mesenchymal stem cell osteogenesis by transforming growth factor-?茁1. [J]. Journal of Chongqing Medical University 38(5):621-625(2014)
DOI：

Li Ruidong, DENG Zhongliang, Wang Pan, et al. Promotion of bone morphogenetic protein 9-induced mesenchymal stem cell osteogenesis by transforming growth factor-?茁1. [J]. Journal of Chongqing Medical University 38(5):621-625(2014) DOI：

摘要

目的：探讨转化生长因子?茁1（transforming growth factor-?茁1，TGF-?茁1）在骨形态发生蛋白9（bone morphogenetic protein 9，BMP9）诱导间充质干细胞（mesenchymal stem cell，MSC）成骨分化过程中的作用。方法：应用鼠MSC C3H10T1/2，以重组腺病毒法将BMP9导入，施加TGF-?茁1处理，建立BMP9联合TGF-β1诱导MSC成骨分化模型。改良SEAP化学发光法检测碱性磷酸酶（alkaline phosphatase，ALP）活性；茜素红S染色进行钙盐沉积检测；实时荧光定量PCR检测成骨相关基因Ⅰa2型胶原（collagenⅠa2，COLⅠa2）、骨桥素（osteopotin，OPN）、骨钙素（osteocalcin，OCN）的mRNA转录表达水平，免疫细胞化学法检测细胞内OPN、OCN表达变化。荧光素酶报告基因法检测信号通路的Smad水平。结果：TGF-?茁1与BMP9联合处理较BMP9单独处理能更早更多地提高ALP活性和引起钙盐沉积（F处理=18 782.10，P=0.000），持续处理达17 d后不再显示明显差异，TGF-?茁1单独处理与对照组间无明显差异（F=1.03，P=0.318）。TGF-?茁1与BMP9联合处理较BMP9单独处理可使COLⅠa2、OPN、OCN mRNA转录水平明显增高（COLⅠa2的F处理=250.30，P=0.000；OPN 的F处理=795.64，P=0.000；OCN的F处理=206.55，P=0.000），COLⅠa2增高发生较早和显著（F=250.30，P=0.000）；细胞内OPN、OCN蛋白阳性表达显著增强。TGF-β1联合BMP9处理能刺激信号通路相应的Smad荧光素报告活性（?字2=32.84，P=0.000）。结论：在BMP9诱导MSC成骨分化中，联合应用TGF-?茁1具有促进成骨作用，BMP9与TGF-?茁1在成骨分化中可能存在互补效应。

Abstract

Objective：To investigate the effect of transforming growth factor-β1（TGF-β1） on bone morphogenetic protein 9（BMP9）-induced mesenchymal stem cells（MSCs） osteogenic differentiation. Methods：C3H10T1/2 cell line infected with BMP9 adenovirus and added with TGF-β1 protein was used to create the osteogenic model. The modified SEAP chemoluminescence assay was used for al－kaline phosphatase（ALP） activity. Alizarin red staining was used for mineralization. Real-time PCR and immunocytochemistry were used for bone markers collagen typeⅠa2（COLⅠa2），osteopotin（OPN） and osteocalcin（OCN）. Luciferase reporter assay was used for detecting the Smad expression. Results：BMP9 combined with TGF-β1 induced more ALP activities and mineralization（F=18 782.10，P=0.000） than single BMP9. However，there was no difference between single TGF-β1 group and single GFP group after treating con－secutively for 17 d（F=1.03，P=0.318）. mRNA expression of OPN，OCN and COLⅠa2 was increased in BMP9 combined with TGF-β1 group than in single BMP9 group（FCOLⅠa2=250.30，P=0.000；FOPN=795.64，P=0.000；FOCN=206.55，P=0.000）. BMP9 combined with TGF-β1 increased COLⅠa2 more significantly at earlier stage（F=250.30，P=0.000） and increased the protein expression of OPN and OCN in vitro. BMP9 combined with TGF-β1 stimulated the activity of luciferase reporter of related Smads（?字2=32.84，P=0.000）. Conclu－sions：TGF-β1 promotes BMP9-induced osteogenic differentia－tion in MSCs. TGF-β1 and BMP9 might have synergistic effects.

关键词

骨形成蛋白9转化成因子?茁1间充质干细胞分化成骨

Keywords

bone morphogenetic protein 9transforming growth factor-β1mesenchymal stem cellsdifferentiationosteogenesis

references

Views

438

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Research advances in the therapeutic effect of mesenchymal stem cell exosomes on pulmonary hypertension

Influence of microenvironment of normal neurons and neurons expressing seizure-like discharge induced by free-magnesium extracellular fluid culture on the differentiation of oligodendrocyte precursor cells

The observation of CD44 on differentiation and vascular invasion in hepatocellular carcinoma

Effects of environment in vivo and in vitro on the differentiation of bone mesenchymal stem cells into neural cells

5-azacytidine induce bone marrow mesenchymal stem cell differentiation into cardiomyocyte-like cells in vitro

Related Author

Zhang Qingqing

Ge Rili

Ma Lan

Wang Yue

Jiang Li

Liu Liming

Yang Xia

Chen Hengsheng

Related Institution

Research Center for High Altitude Medicine， Qinghai University；Key Laboratory of High Altitude Medicine， Ministry of Education；Key Laboratory of Application and Foundation for High Altitude Medicine Research in Qinghai Province

Department of Neurology,the Children’s Hospital,Chongqing Medical University

重庆医科大学病理教研

重庆医科大学病理教研室

室重庆

⁰