Effect of miR-138-5p targeting HIF-1α on proliferation and invasion and metastasis of human leukemia K562 cells

GUO Tao; JI Dongmei; LI Yanping; LU Yang; YI Li; WANG Xiaohong

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Effect of miR-138-5p targeting HIF-1α on proliferation and invasion and metastasis of human leukemia K562 cells

更新时间：2022-06-24

- Effect of miR-138-5p targeting HIF-1α on proliferation and invasion and metastasis of human leukemia K562 cells
- null Issue 4, Pages: 272(2020)
- 作者机构：
  
  朝阳市中心医院血液内科,辽宁,朝阳,122000
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GUO Tao, JI Dongmei, LI Yanping, et al. Effect of miR-138-5p targeting HIF-1α on proliferation and invasion and metastasis of human leukemia K562 cells. [J]. null (4):272(2020)
DOI：

GUO Tao, JI Dongmei, LI Yanping, et al. Effect of miR-138-5p targeting HIF-1α on proliferation and invasion and metastasis of human leukemia K562 cells. [J]. null (4):272(2020) DOI：

摘要

目的探索miR-138-5p与缺氧诱导因子-1α(HIF-1α)对人白血病K562细胞增殖和侵袭转移的影响。方法以体外培养的人白血病K562细胞为研究对象

将其分为K562组、miR-138 mimic组、pc-HIF-1α组和mimic+pc-HIF-1α组。荧光素酶报告实验确认miR-138-5p与HIF-1α的靶向关系;qRT-PCR法检测miR-138-5p和HIF-1α的mRNA水平;Western blot检测HIF-1α的蛋白水平;CCK-8检测细胞存活率;Transwell检测细胞侵袭能力;划痕实验检测细胞迁移能力;Western blot检测增殖和上皮间质转化(EMT)相关蛋白表达水平。结果 miR-138-5p能靶向HIF-1α

与K562组相比

miR-138 mimic组HIF-1α的mRNA和蛋白水平均显著较低(P<0.01)

而pc-HIF-1α组HIF-1α的蛋白水平则显著较高(P<0.01);同时

与pc-HIF-1α组相比

mimic+pc-HIF-1α组HIF-1α的蛋白表达显著较低(P<0.01)。与K562组相比

miR-138 mimic组细胞增殖倍数、细胞迁移和侵袭能力均显著较低(P<0.01)

pc-HIF-1α组则显著较高(P<0.01);与pc-HIF-1α组相比

mimic+pc-HIF-1α组细胞增殖倍数、细胞迁移和侵袭能力均显著较低(P<0.01)。同时

mimic+pc-HIF-1α能部分逆转pc-HIF-1α对肿瘤细胞增殖和EMT能力的上调作用(P<0.01)。结论miR-138-5p可通过靶向HIF-1α

进而降低人白血病K652细胞增殖和侵袭转移能力。

Abstract

关键词

白血病慢性粒细胞白血病miR-138-5pHIF-1α增殖上皮间质化

Keywords

leukemiachronic granulocytic leukemiamiR-138-5pHIF-1αproliferationepithelial mesenchymal transformation

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