Objective To investigate the impact of 52 Hz small molecule water on components of urine and urine over-saturation through glycol-induced kidney stones model of rat so as to explore the mechanism of inhibiting stone formation.Methods 60 female SD rats were randomly divided into four groups

namely

ordinary water+glycol feeding group（Group A）

small molecule water+glycol feeding group（Group B）

ordinary water feeding group（Group C）

small molecule water feeding group（Group D）

15 rats in each group.All rats were under the same rearing condition and they were free to drink and eat.After 40 days

all groups were tested blood and urine biochemical index

their urine ionic activities were calculated with urinary biochemical results and stone formation was observed.Results In Group A

rats′urine volume of 24 hours was（6.70±1.47）mL

higher than Groups B

C and D

but the differences were not statistically significant.Urinary calcium of Group A was（4.31±0.34）mmol/L

higher than Groups B

C and D

and the differences were statistically significant（P<0.05）.Urinary oxalate of Group A was（1.24±0.31）mmol/L

higher than Groups B

C and D

and the differences were statistically significant（P<0.05）.Urinary citrate of Group A was（7.76±1.15）mmol/L and that of Group B was（8.52±1.34）mmol/L;the differences between Group A and Groups C and D were statistically significant（P<0.05）and there were no significant differences between Group B and Groups C and D.Urinary magnesium concentration of Group A was the lowest

（6.58±0.78）mmol/L

and the differences between Group A and Groups B

C and D were statistically significant（P<0.05）;urinary magnesium concentration of Group B was（7.52±1.18）mmol/L

and the differences between Group B and Groups C and D were statistically significant（P<0.05）.There were no significant differences among four groups in serum calcium

serum magnesium

urea

serum phosphorus.The levels of creatinine and urea nitrogen in rats of Group A were higher than Groups B

C and D

and the differences were statistically significant（P<0.05）.Conclusion 52 Hz small molecule water can increase the solubility of ions in urine and it can reduce the concentration of ions promoting stone formation and increase the concentration of ions inhibiting stone formation in order to inhibit the formation of stones.