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Th1/Th2促进早发性重度子痫前期胎盘滋养细胞焦亡
Increased ratio of Th1/Th2 cells promotes pyroptosis of placental trophoblast cells in early-onset severe preeclampsia
- 陆军军医大学学报 2022年44卷第3期 页码：246-252
- 作者机构：
050051 石家庄，石家庄市第四医院检验科
- 作者简介：
[刘力，E-mail：489569712@qq.comLIU Li, E-mail: 489569712@qq.com
- 基金信息：河北省医学科学研究重点课题计划(20201380)
DOI：10.16016/j.2097-0927.202107142
中图分类号： R321.4;R392.12;R714.245收稿：2021-07-23，
修回：2021-11-04，
纸质出版：2022-02-15
- 稿件说明：
移动端阅览
王海娇, 王毅, 何红美, 等. Th1/Th2促进早发性重度子痫前期胎盘滋养细胞焦亡[J]. 陆军军医大学学报, 2022,44(3):246-252.
Haijiao WANG, Yi WANG, Hongmei HE, et al. Increased ratio of Th1/Th2 cells promotes pyroptosis of placental trophoblast cells in early-onset severe preeclampsia[J]. Journal of Army Medical University, 2022, 44(3): 246-252.
王海娇, 王毅, 何红美, 等. Th1/Th2促进早发性重度子痫前期胎盘滋养细胞焦亡[J]. 陆军军医大学学报, 2022,44(3):246-252. DOI： 10.16016/j.2097-0927.202107142.
Haijiao WANG, Yi WANG, Hongmei HE, et al. Increased ratio of Th1/Th2 cells promotes pyroptosis of placental trophoblast cells in early-onset severe preeclampsia[J]. Journal of Army Medical University, 2022, 44(3): 246-252. DOI： 10.16016/j.2097-0927.202107142.
摘要
目的
2
研究Th1/Th2对早发性重度子痫前期(early-onset severe preeclampsia，EOSP)胎盘滋养细胞焦亡的影响。
方法
2
收集2018年6月至2019年12月本院妇产科确诊为EOSP的55例临床资料为子痫前期组，选取同期本院产检正常孕妇30例为正常组。应用流式细胞仪检测各组孕妇静脉血中Th1和Th2细胞的比例，应用ELISA检测血清中细胞焦亡相关因子IL-1β、IL-18的含量、应用TUNEL染色观察胎盘组织焦亡的情况；应用免疫荧光染色观察胎盘组织特异性切割关键焦亡底物蛋白消皮素D(Gasdermin D，GSDMD)的表达；应用Western blot检测Caspase-1、NLRP3、接头蛋白(ASC)、GSDMD的蛋白表达。
结果
2
流式结果显示，子痫前期组Th1/Th2细胞的比例与正常组相比较明显升高；ELISA结果显示，子痫前期组血清中细胞焦亡相关因子IL-1β、IL-18的含量明显升高；TUNEL染色结果显示，子痫前期组可见大量的类似凋亡细胞，明显多于正常组；免疫荧光染色GSDMD发现，子痫前期组的表达量明显多于对照组；Western blot结果显示，Caspase-1、NLRP3、ASC、GSDMD的蛋白表达明显高于正常组。
结论
2
早发性重度子痫前期时，Th1/Th2的比例增高促进了胎盘滋养细胞发生细胞焦亡。
Abstract
Objective
2
To investigate the effect of Th1/Th2 ratio on pyroptosis of placental trophoblast cells in early-onset severe preeclampsia.
Methods
2
The clinical data of 55 cases of early-onset severe preeclampsia diagnosed in our hospital from June 2018 to December 2019 were collected as the preeclampsia group
and another 30 healthy pregnant women in our hospital during the same period were subjected as the normal control. Flow cytometry was used to detect the proportion of Th1 and Th2 cells in venous blood of the subjects; ELISA was adopted to measure the contents of pyroptosis-related factors IL-1β and IL-18 in serum. The placental tissue pyroptosis and the expression of GSDMD protein in placental tissue were observed by TUNEL staining and immunofluorescence staining respectively. The protein levels of Caspase-1
NLRP3
ASC and GSDMD were determined by Western blotting.
Results
2
Flow cytometry showed that the ratio of Th1/Th2 cells was significantly increased in the preeclampsia group. ELISA results indicated that the contents of IL-1β and IL-18 in the serum were remarkably higher in the preeclampsia group than the normal group (
P
<
0.01). TUNEL staining and immunofluorescence staining also confirmed a large number of pyroptosis-like cells and high level of GSDMD in the preeclampsia group; Western blotting demonstrated that the protein levels of Caspase-1
NLRP3
ASC and GSDMD were all notably elevated
as compared with the normal group.
Conclusion
2
In early-onset severe preeclampsia
the increased Th1/Th2 ratio promotes pyroptosis of placental trophoblast cells.
关键词
Keywords
references
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