CHEN Yong, et al. Content determination of Puerarin in Xinkeshu Capsule by HPLC[J]. Journal of Chongqing Medical University, 2005,(5). DOI: 10.13406/j.cnki.cyxb.2005.05.023.
Objective:To establish a HPLC method for the content determination of Puerarin in Xinkeshu capsule.Methods:The chromatography condition included C18 silicane-bonded silica gel column(250mm×4.6mm
5μm)
MeOH-1.5%HAc-CHCI
3
(25:74:1) mixture as the mobile phase
detection wavelength at 250nm
1.1ml/min of flow rate.Results:Puerarin was completely separated and no interference was found. The average recovery was 99.2%
RSD was 0.87%(n=5).Conclusion:The method is accurate
quick
sensitive and replicable
which is suitable for the quality control of Xinkeshu capsule.