抵抗素对肝细胞葡萄糖激酶和磷酸烯醇式丙酮酸激酶活性的影响

李超林; 龚明; 肖谦; 黄昶全; 彭惠民

doi:10.13406/j.cnki.cyxb.2005.05.008

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抵抗素对肝细胞葡萄糖激酶和磷酸烯醇式丙酮酸激酶活性的影响

更新时间：2024-01-17

- 抵抗素对肝细胞葡萄糖激酶和磷酸烯醇式丙酮酸激酶活性的影响
- Effect of resistin on activity of glucoskinase and phosphoenolpyruvate carboxykinase in cultured liver cells
- 重庆医科大学学报 2005年第5期
- 作者机构：
  
  1. 重庆医科大学附属第一医院老年科
  2. 重庆医科大学基础医学院生物教研室,重庆,400016
  3. 重庆医科大学附属第一医院老年科重庆医科大学基础医学院生物教研室,重庆,400016
- 作者简介：
- 基金信息：
  
  重庆市卫生局科研基金资助（03-2-109）
- DOI：10.13406/j.cnki.cyxb.2005.05.008
  中图分类号： R587.1
- 纸质出版日期：2005，
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[1]李超林,龚明,肖谦,黄昶全,彭惠民.抵抗素对肝细胞葡萄糖激酶和磷酸烯醇式丙酮酸激酶活性的影响[J].重庆医科大学学报,2005(05):34-36.

LI Chaolin, et al. Effect of resistin on activity of glucoskinase and phosphoenolpyruvate carboxykinase in cultured liver cells[J]. Journal of Chongqing Medical University, 2005,(5).
[1]李超林,龚明,肖谦,黄昶全,彭惠民.抵抗素对肝细胞葡萄糖激酶和磷酸烯醇式丙酮酸激酶活性的影响[J].重庆医科大学学报,2005(05):34-36. DOI： 10.13406/j.cnki.cyxb.2005.05.008.

LI Chaolin, et al. Effect of resistin on activity of glucoskinase and phosphoenolpyruvate carboxykinase in cultured liver cells[J]. Journal of Chongqing Medical University, 2005,(5). DOI： 10.13406/j.cnki.cyxb.2005.05.008.

摘要

目的:观察抵抗素（Resistin）对肝细胞糖代谢关键酶葡萄糖激酶（GK）和磷酸烯醇式丙酮酸激酶（PEPCK）活性的影响及剂量效应。方法:正常大鼠的肝细胞

以不加Resistin与加不同剂量的Resistin培养24h

用6-磷酸葡萄糖脱氢酶偶联比色法、乳酸脱氢酶偶联比色法检测不同剂量Resistin对GK和PKPCK的影响。结果:Resistin浓度为10nM/L、50nM/L时

GK活性与空白对照组无明显差异

PEPCK活性明显升高（P<0.05）;当Resistin浓度上升为100nmol/L后

GK活性较空白对照组明显下降（P<0.01）

而PEPCK活性升高更明显（P<0.01）。结论:抵抗素浓度升高使大鼠肝细胞GK活性下降

PEPCK活性升高

从而抑制糖酵解促进糖异生

升高血糖。上述两种酶的活性与抵抗素浓度呈现不同的剂量依赖关系。

Abstract

Objective:To observe the effect of resistin on activity of glucoskinase(GK) and phosphoenolpyruvate carboxykinase(PEPCK) in cultured rat liver cells.Methods:Rat liver cells were incubated with resistin of different doses(ranging from 10nM/L to 100nM/L).Controled liver cells were invubated without resistin.Glucose-6-phosphate dehydrogenase couple chromatometry and lactate dehydrogenase couple chromatometry were used to determine activity of GK and PEPCK.Results:Compared with the control group

treated with resistin of 10nM/L and 50nM/L for 24h had no significant effect on activity of GK

but activity of PKPCK increased significantly(P<0.05).At the dose of 100nM/L

resistin inhabited activity of GK markedly(P<0.01)

while activity of PKPCK increased more significantly(P<0.01).Conclusion:The increase of Resistin leads to the decrease of activity of GK and increase of activity of PEPCK in rat liver cells

hence inhabite glycolysis and promote glyconeogenesis and increase blood glucose as well.The activity of two enzymes shows dose dependent relation with resistin.

关键词

肝细胞葡萄糖激酶磷酸烯醇式丙酮酸激酶抵抗素

Keywords

Liver cellsGlucoskinasePhosphoemolpyruvate carboxykinaseresistin

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