Objective:To explore the optimal transfection conditions in which the concentrated virus supernatant transfer the foreign mdr-1 gene into hematopoietic cells of rabbit bone marrow

and test the expression and function of the mdr-1 gene in cells.Methods:An amphotropic virus producer cell line

PA317-HaMDR1/A1 which contains a full-length cDNA of human mdr-1 gene

was screened with colchicine(90ng/ml)

and the supernatant was collected and concentrated.The bone marrow cells of the New Zealand rabbits were gathered and the mononuclear cells which contain many hematopoietic cells were enriched.The hematopoietic cells were cocultivated with concentrated virus supernatant and interleukins.The transfection rate was determined by SP immunohistochemical methods;the integration of mdr-1 gene in rabbit mononuclear cells was tested by PCR method

and the physiological function of mdr-1 gene was tested by DNR extrusion test.Results:After screening by colchicine

the expression of P-glycoprotein(P-gp) of PA317-HaMDR1/A1 was enhanced.The foreign mdr-1 gene was transferred into hematopoietic cells of rabbit bone marrow successfully

and the transduction rate of 2 days

4days and 6 days were 22%

37%

and 39% respectively.However

the state of the 4 days was the best.The transferred mdr-1 gene had its physiological function.Conclusion:The foreign mdr-1 gene can be transferred into hematopoietic cells of rabbit bone marrow successfully by concentrated virus supernatant transfection method

and the transferring gene can be expressed stably and effectively.The study has provided a basis for the further research on chemoprotection experiment of the mdr-1 gene transferred into the bone marrow cells.