In vitro comparison of human plasma-based and self-assembled tissue-engineered skin substitutes: two different manufacturing processes for the treatment of deep and difficult to heal injuries

Álvaro Sierra-Sánchez; Brice Magne; Etienne Savard; Christian Martel; Karel Ferland; Martin A Barbier; Anabelle Demers; Danielle Larouche; Salvador Arias-Santiago; Lucie Germain

doi:10.1093/burnst/tkad043

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In vitro comparison of human plasma-based and self-assembled tissue-engineered skin substitutes: two different manufacturing processes for the treatment of deep and difficult to heal injuries

更新时间：2024-02-04

- In vitro comparison of human plasma-based and self-assembled tissue-engineered skin substitutes: two different manufacturing processes for the treatment of deep and difficult to heal injuries
- In vitro comparison of human plasma-based and self-assembled tissue-engineered skin substitutes: two different manufacturing processes for the treatment of deep and difficult to heal injuries
- Burns & Trauma 2023年11卷第1期
- 作者机构：
  
  1. LOEX Tissue Engineering Laboratory and Department of Surgery
  2. Université Laval
  3. CHU de Québec – Université Laval Research Center, Division of Regenerative Medicine
  4. Unidad de Producción Celular e Ingeniería Tisular (UPCIT), Virgen de las Nieves University Hospital, ibs
  5. Department of Dermatology, Virgen de las Nieves University Hospital
  6. Department of Dermatology, Faculty of Medicine, University of Granada
- 作者简介：
- 基金信息：
  
  This study has been funded by the Instituto de Salud Carlos III through the project PI17/02083 (co-funded by the European Regional Development Fund “A way to make Europe”), the Regional Government of Andalusia (PIGE-0242-2019), the Canadian Institutes for Health Research (CIHR) (FDN-143213 and IC-132948), the Fondation des Pompiers du Québec pour les Grands Brûlés (FPQGB) and the Quebec Network for Cell, Tissue and Gene Therapy—ThéCell (a thematic network supported by the Fonds de Recherche du Québec—Santé [FRQS]).
- DOI：10.1093/burnst/tkad043
  中图分类号：
- 纸质出版日期：2023，
  
  网络出版日期：2023-10-31，
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Álvaro Sierra-Sánchez, Brice Magne, Etienne Savard, 等. In vitro comparison of human plasma-based and self-assembled tissue-engineered skin substitutes: two different manufacturing processes for the treatment of deep and difficult to heal injuries[J]. Burns & Trauma, 2023,11(1).

Álvaro Sierra-Sánchez, Brice Magne, Etienne Savard, Christian Martel, Karel Ferland, Martin A Barbier, Anabelle Demers, Danielle Larouche, Salvador Arias-Santiago, Lucie Germain, In vitro comparison of human plasma-based and self-assembled tissue-engineered skin substitutes: two different manufacturing processes for the treatment of deep and difficult to heal injuries, Burns & Trauma, Volume 11, 2023, tkad043, https://doi.org/10.1093/burnst/tkad043
Álvaro Sierra-Sánchez, Brice Magne, Etienne Savard, 等. In vitro comparison of human plasma-based and self-assembled tissue-engineered skin substitutes: two different manufacturing processes for the treatment of deep and difficult to heal injuries[J]. Burns & Trauma, 2023,11(1). DOI： 10.1093/burnst/tkad043.

Álvaro Sierra-Sánchez, Brice Magne, Etienne Savard, Christian Martel, Karel Ferland, Martin A Barbier, Anabelle Demers, Danielle Larouche, Salvador Arias-Santiago, Lucie Germain, In vitro comparison of human plasma-based and self-assembled tissue-engineered skin substitutes: two different manufacturing processes for the treatment of deep and difficult to heal injuries, Burns & Trauma, Volume 11, 2023, tkad043, https://doi.org/10.1093/burnst/tkad043 DOI：

摘要

Abstract

BackgroundThe aim of this in vitro study was to compare side-by-side two models of human bilayered tissue-engineered skin substitutes (hbTESSs) designed for the treatment of severely burned patients. These are the scaffold-free self-assembled skin substitute (SASS) and the human plasma-based skin substitute (HPSS).MethodsFibroblasts and keratinocytes from three humans were extracted from skin biopsies (N = 3) and cells from the same donor were used to produce both hbTESS models. For SASS manufacture

keratinocytes were seeded over three self-assembled dermal sheets comprising fibroblasts and the extracellular matrix they produced (n = 12)

while for HPSS production

keratinocytes were cultured over hydrogels composed of fibroblasts embedded in either plasma as unique biomaterial (Fibrin)

plasma combined with hyaluronic acid (Fibrin-HA) or plasma combined with collagen (Fibrin-Col) (n/biomaterial = 9). The production time was 46–55 days for SASSs and 32–39 days for HPSSs. Substitutes were characterized by histology

mechanical testing

PrestoBlue™-assay

immunofluorescence (Ki67

Keratin (K) 10

K15

K19

Loricrin

type IV collagen) and Western blot (type I and IV collagens).ResultsThe SASSs were more resistant to tensile forces (p-value < 0.01) but less elastic (p-value < 0.001) compared to HPSSs. A higher number of proliferative Ki67+ cells were found in SASSs although their metabolic activity was lower. After epidermal differentiation

no significant difference was observed in the expression of K10

K15

K19 and Loricrin. Overall

the production of type I and type IV collagens and the adhesive strength of the dermal-epidermal junction was higher in SASSs.ConclusionsThis study demonstrates

for the first time

that both hbTESS models present similar in vitro biological characteristics. However

mechanical properties differ and future in vivo experiments will aim to compare their wound healing potential.

关键词

Keywords

BiomaterialBurnFibrinHuman plasmaSelf-assemblySkin substituteTissue engineering

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