A volar skin excisional wound model for in situ evaluation of multiple-appendage regeneration and innervation

Huanhuan Gao; Yiqiong Liu; Ziwei Shi; Hongliang Zhang; Mengyang Wang; Huating Chen; Yan Li; Shaifei Ji; Jiangbing Xiang; Wei Pi; Laixian Zhou; Yiyue Hong; Lu Wu; Aizhen Cai; Xiaobing Fu; Xiaoyan Sun

doi:10.1093/burnst/tkad027

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A volar skin excisional wound model for in situ evaluation of multiple-appendage regeneration and innervation

更新时间：2023-08-29

- A volar skin excisional wound model for in situ evaluation of multiple-appendage regeneration and innervation
- A volar skin excisional wound model for in situ evaluation of multiple-appendage regeneration and innervation
- Burns & Trauma 2023年11卷第1期
- 作者机构：
  
  1. Research Center for Tissue Repair and Regeneration Affiliated to Medical Innovation Research Department and 4th Medical Center
  2. Chinese Academy of Medical Sciences
  3. Institute of Chemistry, Chinese Academy of Sciences
  4. Research Institute of General Surgery
  5. the First Medical Center, PLA General Hospital
- 作者简介：
- 基金信息：
  
  This work was supported in part by the National Nature Science Foundation of China [92268206, 81830064];the CAMS Innovation Fund for Medical Sciences [CIFMS, 2019-I2M-5-059];the Military Medical Research Projects [145AKJ260015000X;2022-JCJQ-ZB-09600, 2020-JCJQ-ZD-256-021];the Military Medical Research and Development Projects [AWS17J005, 2019-126];and the Specific Research Fund of The Innovation Platform for Academicians of Hainan Province [YSPTZX202317].
- DOI：10.1093/burnst/tkad027
  中图分类号：
- 纸质出版日期：2023，
  
  网络出版日期：2023-6-29，
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Huanhuan Gao, Yiqiong Liu, Ziwei Shi, 等. A volar skin excisional wound model for in situ evaluation of multiple-appendage regeneration and innervation[J]. Burns & Trauma, 2023,11(1).

Huanhuan Gao, Yiqiong Liu, Ziwei Shi, Hongliang Zhang, Mengyang Wang, Huating Chen, Yan Li, Shaifei Ji, Jiangbing Xiang, Wei Pi, Laixian Zhou, Yiyue Hong, Lu Wu, Aizhen Cai, Xiaobing Fu, Xiaoyan Sun, A volar skin excisional wound model for in situ evaluation of multiple-appendage regeneration and innervation, Burns & Trauma, Volume 11, 2023, tkad027, https://doi.org/10.1093/burnst/tkad027
Huanhuan Gao, Yiqiong Liu, Ziwei Shi, 等. A volar skin excisional wound model for in situ evaluation of multiple-appendage regeneration and innervation[J]. Burns & Trauma, 2023,11(1). DOI： 10.1093/burnst/tkad027.

Huanhuan Gao, Yiqiong Liu, Ziwei Shi, Hongliang Zhang, Mengyang Wang, Huating Chen, Yan Li, Shaifei Ji, Jiangbing Xiang, Wei Pi, Laixian Zhou, Yiyue Hong, Lu Wu, Aizhen Cai, Xiaobing Fu, Xiaoyan Sun, A volar skin excisional wound model for in situ evaluation of multiple-appendage regeneration and innervation, Burns & Trauma, Volume 11, 2023, tkad027, https://doi.org/10.1093/burnst/tkad027 DOI：

摘要

Abstract

BackgroundPromoting rapid wound healing with functional recovery of all skin appendages is the main goal of regenerative medicine. So far current methodologies

including the commonly used back excisional wound model (BEWM) and paw skin scald wound model

are focused on assessing the regeneration of either hair follicles (HFs) or sweat glands (SwGs). How to achieve de novo appendage regeneration by synchronized evaluation of HFs

SwGs and sebaceous glands (SeGs) is still challenging. Here

we developed a volar skin excisional wound model (VEWM) that is suitable for examining cutaneous wound healing with multiple-appendage restoration

as well as innervation

providing a new research paradigm for the perfect regeneration of skin wounds.MethodsMacroscopic observation

iodine–starch test

morphological staining and qRT-PCR analysis were used to detect the existence of HFs

SwGs

SeGs and distribution of nerve fibres in the volar skin. Wound healing process monitoring

HE/Masson staining

fractal analysis and behavioral response assessment were performed to verify that VEWM could mimic the pathological process and outcomes of human scar formation and sensory function impairment.ResultsHFs are limited to the inter-footpads. SwGs are densely distributed in the footpads

scattered in the IFPs. The volar skin is richly innervated. The wound area of the VEWM at 1

7 and 10 days after the operation is respectively 89.17% ± 2.52%

71.72% ± 3.79%

55.09 % ± 4.94% and 35.74% ± 4.05%

and the final scar area accounts for 47.80% ± 6.22% of the initial wound. While the wound area of BEWM at 1

7 and 10 days after the operation are respectively 61.94% ± 5.34%

51.26% ± 4.89%

12.63% ± 2.86% and 6.14% ± 2.84%

and the final scar area accounts for 4.33% ± 2.67% of the initial wound. Fractal analysis of the post-traumatic repair site for VEWM vs human was performed: lacunarity values

0.040 ± 0.012 vs 0.038 ± 0.014; fractal dimension values

1.870 ± 0.237 vs 1.903 ± 0.163. Sensory nerve function of normal skin vs post-traumatic repair site was assessed: mechanical threshold

1.05 ± 0.52 vs 4.90 g ± 0.80; response rate to pinprick

100% vs 71.67% ± 19.92%

and temperature threshold

50.34°C ± 3.11°C vs 52.13°C ± 3.54°C.ConclusionsVEWM closely reflects the pathological features of human wound healing and can be applied for skin multiple-appendages regeneration and innervation evaluation.

关键词

Keywords

Wound healingAnimal modelMultiple appendagesInnervationRegeneration evaluationHair folliclesSweat glandSebaceous gland

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