Single-cell RNA-seq and bulk-seq identify RAB17 as a potential regulator of angiogenesis by human dermal microvascular endothelial cells in diabetic foot ulcers

Hengyu Du; Shenghong Li; Jinqiang Lu; Lingzhi Tang; Xiao Jiang; Xi He; Jiaji Liang; Xuan Liao; Taixing Cui; Yuesheng Huang; Hongwei Liu

doi:10.1093/burnst/tkad020

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Single-cell RNA-seq and bulk-seq identify RAB17 as a potential regulator of angiogenesis by human dermal microvascular endothelial cells in diabetic foot ulcers

更新时间：2023-08-29

- Single-cell RNA-seq and bulk-seq identify RAB17 as a potential regulator of angiogenesis by human dermal microvascular endothelial cells in diabetic foot ulcers
- Single-cell RNA-seq and bulk-seq identify RAB17 as a potential regulator of angiogenesis by human dermal microvascular endothelial cells in diabetic foot ulcers
- Burns & Trauma 2023年11卷第1期
- 作者机构：
  
  1. Department of Plastic Surgery of the First Affiliated Hospital of Jinan University, Institute of New Technology of Plastic Surgery of Jinan University, Key Laboratory of Regenerative Medicine of Ministry of Education
  2. Dalton Cardiovascular Research Center
  3. University of Missouri, School of Medicine
  4. Institute of Wound Repair and Regeneration Medicine, Southern University of Science and Technology School of Medicine, and Department of Wound Repair, Southern University of Science and Technology Hospital
- 作者简介：
- 基金信息：
  
  This work was supported by National Nature and Science Foundation of P.R. China (No. 81871563, 82102345), the Medical Research Foundation of Guangdong Province (A2021165), the Fundamental Research Funds for the Central Universities (21619350), Guangdong Foundation for Basic and Applied Basic Research (2019A1515110161), Funding by Science and Technology Projects in Guangzhou (202201020002, 202201020004, 202201020468), the Clinical Frontier Technology Program of the First Affiliated Hospital of Jinan University, China (No. JNU1AF-CFTP-2022-a01231, No. JNU1AF-CFTP-2022-a01208) and Guangdong Provincial Key Areas R&D Programs (2022B1111080007).
- DOI：10.1093/burnst/tkad020
  中图分类号：
- 纸质出版日期：2023，
  
  网络出版日期：2023-8-18，
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Hengyu Du, Shenghong Li, Jinqiang Lu, 等. Single-cell RNA-seq and bulk-seq identify RAB17 as a potential regulator of angiogenesis by human dermal microvascular endothelial cells in diabetic foot ulcers[J]. Burns & Trauma, 2023,11(1).

Hengyu Du, Shenghong Li, Jinqiang Lu, Lingzhi Tang, Xiao Jiang, Xi He, Jiaji Liang, Xuan Liao, Taixing Cui, Yuesheng Huang, Hongwei Liu, Single-cell RNA-seq and bulk-seq identify RAB17 as a potential regulator of angiogenesis by human dermal microvascular endothelial cells in diabetic foot ulcers, Burns & Trauma, Volume 11, 2023, tkad020, https://doi.org/10.1093/burnst/tkad020
Hengyu Du, Shenghong Li, Jinqiang Lu, 等. Single-cell RNA-seq and bulk-seq identify RAB17 as a potential regulator of angiogenesis by human dermal microvascular endothelial cells in diabetic foot ulcers[J]. Burns & Trauma, 2023,11(1). DOI： 10.1093/burnst/tkad020.

Hengyu Du, Shenghong Li, Jinqiang Lu, Lingzhi Tang, Xiao Jiang, Xi He, Jiaji Liang, Xuan Liao, Taixing Cui, Yuesheng Huang, Hongwei Liu, Single-cell RNA-seq and bulk-seq identify RAB17 as a potential regulator of angiogenesis by human dermal microvascular endothelial cells in diabetic foot ulcers, Burns & Trauma, Volume 11, 2023, tkad020, https://doi.org/10.1093/burnst/tkad020 DOI：

摘要

Abstract

BackgroundAngiogenesis is crucial in diabetic wound healing and is often impaired in diabetic foot ulcers (DFUs). Human dermal microvascular endothelial cells (HDMECs) are vital components in dermal angiogenesis; however

their functional and transcriptomic characteristics in DFU patients are not well understood. This study aimed to comprehensively analyse HDMECs from DFU patients and healthy controls and find the potential regulator of angiogenesis in DFUs.MethodsHDMECs were isolated from skin specimens of DFU patients and healthy controls via magnetic-activated cell sorting. The proliferation

migration and tube-formation abilities of the cells were then compared between the experimental groups. Both bulk RNA sequencing (bulk-seq) and single-cell RNA-seq (scRNA-seq) were used to identify RAB17 as a potential marker of angiogenesis

which was further confirmed via weighted gene co-expression network analysis (WGCNA) and least absolute shrink and selection operator (LASSO) regression. The role of RAB17 in angiogenesis was examined through in vitro and in vivo experiments.ResultsThe isolated HDMECs displayed typical markers of endothelial cells. HDMECs isolated from DFU patients showed considerably impaired tube formation

rather than proliferation or migration

compared to those from healthy controls. Gene set enrichment analysis (GSEA)

fGSEA

and gene set variation analysis (GSVA) of bulk-seq and scRNA-seq indicated that angiogenesis was downregulated in DFU-HDMECs. LASSO regression identified two genes

RAB17 and CD200

as characteristic of DFU-HDMECs; additionally

the expression of RAB17 was found to be significantly reduced in DFU-HDMECs compared to that in the HDMECs of healthy controls. Overexpression of RAB17 was found to enhance angiogenesis

the expression of hypoxia inducible factor-1α and vascular endothelial growth factor A

and diabetic wound healing

partially through the mitogen-activated protein kinase/extracellular signal-regulated kinase signalling pathway.ConclusionsOur findings suggest that the impaired angiogenic capacity in DFUs may be related to the dysregulated expression of RAB17 in HDMECs. The identification of RAB17 as a potential molecular target provides a potential avenue for the treatment of impaired angiogenesis in DFUs.

关键词

Keywords

Human dermal microvascular endothelial cellsAngiogenesisDiabetic foot ulcersRAB17Single-cell RNA-seqDiabetic wound healing

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