Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats

Taotao Hu; Shusen Chang; Fang Qi; Zhonghui Zhang; Jiayin Chen; Lingli Jiang; Dali Wang; Chengliang Deng; Kaiyu Nie; Guangchao Xu; Zairong Wei

doi:10.1093/burnst/tkad013

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Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats

更新时间：2023-08-29

- Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats
- Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats
- Burns & Trauma 2023年11卷第1期
- 作者机构：
  
  1. Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical University
  2. The Collaborative Innovation Center of Tissue Damage Repair and Regeneration Medicine of Zunyi medical University
- 作者简介：
- 基金信息：
  
  This work was financially supported by the Innovation Group Major Research Project of Guizhou Province Education Department (No. Qianjiaohe KY [2017]043), the Science and Technology Support Project of Guizhou Province (2020–5012), the PhD Fund of Scientific Research Foundation of the Affiliated Hospital of Zunyi Medical University (2020–03), the National Nature Science Foundation of China (81660325) and the Collaborative Innovation Center of the Chinese Ministry of Education (2020–39), the Master Fund of Scientific Research Foundation of the Affiliated Hospital of Zunyi Medical University (2016–35).
- DOI：10.1093/burnst/tkad013
  中图分类号：
- 纸质出版日期：2023，
  
  网络出版日期：2023-4-27，
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Taotao Hu, Shusen Chang, Fang Qi, 等. Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats[J]. Burns & Trauma, 2023,11(1).

Taotao Hu, Shusen Chang, Fang Qi, Zhonghui Zhang, Jiayin Chen, Lingli Jiang, Dali Wang, Chengliang Deng, Kaiyu Nie, Guangchao Xu, Zairong Wei, Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats, Burns & Trauma, Volume 11, 2023, tkad013, https://doi.org/10.1093/burnst/tkad013
Taotao Hu, Shusen Chang, Fang Qi, 等. Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats[J]. Burns & Trauma, 2023,11(1). DOI： 10.1093/burnst/tkad013.

Taotao Hu, Shusen Chang, Fang Qi, Zhonghui Zhang, Jiayin Chen, Lingli Jiang, Dali Wang, Chengliang Deng, Kaiyu Nie, Guangchao Xu, Zairong Wei, Neural grafts containing exosomes derived from Schwann cell-like cells promote peripheral nerve regeneration in rats, Burns & Trauma, Volume 11, 2023, tkad013, https://doi.org/10.1093/burnst/tkad013 DOI：

摘要

Abstract

BackgroundSchwann cell-like cells (SCLCs)

differentiated from mesenchymal stem cells

have shown promising outcomes in the treatment of peripheral nerve injuries in preclinical studies. However

certain clinical obstacles limit their application. Hence

the primary aim of this study was to investigate the role of exosomes derived from SCLCs (SCLCs-exo) in peripheral nerve regeneration.MethodsSCLCs were differentiated from human amniotic mesenchymal stem cells (hAMSCs) in vitro and validated by immunofluorescence

real-time quantitative PCR and western blot analysis. Exosomes derived from hAMSCs (hAMSCs-exo) and SCLCs were isolated by ultracentrifugation and validated by nanoparticle tracking analysis

WB analysis and electron microscopy. A prefabricated nerve graft was used to deliver hAMSCs-exo or SCLCs-exo in an injured sciatic nerve rat model. The effects of hAMSCs-exo or SCLCs-exo on rat peripheral nerve injury (PNI) regeneration were determined based on the recovery of neurological function and histomorphometric variation. The effects of hAMSCs-exo or SCLCs-exo on Schwann cells were also determined via cell proliferation and migration assessment.ResultsSCLCs significantly expressed the Schwann cell markers glial fibrillary acidic protein and S100. Compared to hAMSCs-exo

SCLCs-exo significantly enhanced motor function recovery

attenuated gastrocnemius muscle atrophy and facilitated axonal regrowth

myelin formation and angiogenesis in the rat model. Furthermore

hAMSCs-exo and SCLCs-exo were efficiently absorbed by Schwann cells. However

compared to hAMSCs-exo

SCLCs-exo significantly promoted the proliferation and migration of Schwann cells. SCLCs-exo also significantly upregulated the expression of a glial cell-derived neurotrophic factor

myelin positive regulators (SRY-box transcription factor 10

early growth response protein 2 and organic cation/carnitine transporter 6) and myelin proteins (myelin basic protein and myelin protein zero) in Schwann cells.ConclusionsThese findings suggest that SCLCs-exo can more efficiently promote PNI regeneration than hAMSCs-exo and are a potentially novel therapeutic approach for treating PNI.

关键词

Keywords

Schwann cell-like cellsExosomesPeripheral nerve injuryMesenchymal stem cellsNerve regeneration

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