Sphingosine-1-phosphate derived from PRP-Exos promotes angiogenesis in diabetic wound healing via the S1PR1/AKT/FN1 signalling pathway

Tianyi Chen; Peiyang Song; Min He; Shunli Rui; Xiaodong Duan; Yu Ma; David G Armstrong; Wuquan Deng

doi:10.1093/burnst/tkad003

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Sphingosine-1-phosphate derived from PRP-Exos promotes angiogenesis in diabetic wound healing via the S1PR1/AKT/FN1 signalling pathway

更新时间：2023-08-29

- Sphingosine-1-phosphate derived from PRP-Exos promotes angiogenesis in diabetic wound healing via the S1PR1/AKT/FN1 signalling pathway
- Sphingosine-1-phosphate derived from PRP-Exos promotes angiogenesis in diabetic wound healing via the S1PR1/AKT/FN1 signalling pathway
- Burns & Trauma 2023年11卷第1期
- 作者机构：
  
  1. Chongqing University Central Hospital, School of Medicine, Chongqing University
  2. The Affiliated Hospital of Southwest Medical University
  3. Keck School of Medicine of University of Southern California
- 作者简介：
- 基金信息：
  
  This study was supported by the Chongqing Youth High-end Talent Studio (Grant No. ZQNYXGDRCGZS2021008), the fund of Sichuan Provincial Western Psychiatric Association’s CSPC LEADING Scientific Research Project (Grant No. WL2021002) and the Natural Science Foundation of Chongqing Municipal Science and Technology Bureau (CSTB2022NSCQ-MSX0489) awarded to WD and SR. This study is partially supported by National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases Award Number 1R01124789-01A1 and National Science Foundation (NSF) Center to Stream Healthcare in Place (#C2SHiP) CNS Award Number 2052578 awarded to DGA.
- DOI：10.1093/burnst/tkad003
  中图分类号：
- 纸质出版日期：2023，
  
  网络出版日期：2023-5-23，
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Tianyi Chen, Peiyang Song, Min He, 等. Sphingosine-1-phosphate derived from PRP-Exos promotes angiogenesis in diabetic wound healing via the S1PR1/AKT/FN1 signalling pathway[J]. Burns & Trauma, 2023,11(1).

Tianyi Chen, Peiyang Song, Min He, Shunli Rui, Xiaodong Duan, Yu Ma, David G Armstrong, Wuquan Deng, Sphingosine-1-phosphate derived from PRP-Exos promotes angiogenesis in diabetic wound healing via the S1PR1/AKT/FN1 signalling pathway, Burns & Trauma, Volume 11, 2023, tkad003, https://doi.org/10.1093/burnst/tkad003
Tianyi Chen, Peiyang Song, Min He, 等. Sphingosine-1-phosphate derived from PRP-Exos promotes angiogenesis in diabetic wound healing via the S1PR1/AKT/FN1 signalling pathway[J]. Burns & Trauma, 2023,11(1). DOI： 10.1093/burnst/tkad003.

Tianyi Chen, Peiyang Song, Min He, Shunli Rui, Xiaodong Duan, Yu Ma, David G Armstrong, Wuquan Deng, Sphingosine-1-phosphate derived from PRP-Exos promotes angiogenesis in diabetic wound healing via the S1PR1/AKT/FN1 signalling pathway, Burns & Trauma, Volume 11, 2023, tkad003, https://doi.org/10.1093/burnst/tkad003 DOI：

摘要

Abstract

BackgroundSphingosine-1-phosphate (S1P)

a key regulator of vascular homeostasis and angiogenesis

is enriched in exosomes derived from platelet-rich plasma (PRP-Exos). However

the potential role of PRP-Exos-S1P in diabetic wound healing remains unclear. In this study

we investigated the underlying mechanism of PRP-Exos-S1P in diabetic angiogenesis and wound repair.MethodsExosomes were isolated from PRP by ultracentrifugation and analysed by transmission electron microscopy

nanoparticle tracking analysis and western blotting. The concentration of S1P derived from PRP-Exos was measured by enzyme-linked immunosorbent assay. The expression level of S1P receptor1–3 (S1PR1–3) in diabetic skin was analysed by Q-PCR. Bioinformatics analysis and proteomic sequencing were conducted to explore the possible signalling pathway mediated by PRP-Exos-S1P. A diabetic mouse model was used to evaluate the effect of PRP-Exos on wound healing. Immunofluorescence for cluster of differentiation 31 (CD31) was used to assess angiogenesis in a diabetic wound model.ResultsIn vitro

PRP-Exos significantly promoted cell proliferation

migration and tube formation. Furthermore

PRP-Exos accelerated the process of diabetic angiogenesis and wound closure in vivo. S1P derived from PRP-Exos was present at a high level

and S1PR1 expression was significantly elevated compared with S1PR2 and S1PR3 in the skin of diabetic patients and animals. However

cell migration and tube formation were not promoted by PRP-Exos-S1P in human umbilical vein endothelial cells treated with shS1PR1. In the diabetic mouse model

inhibition of S1PR1 expression at wounding sites decreased the formation of new blood vessels and delayed the process of wound closure. Bioinformatics analysis and proteomics indicated that fibronectin 1 (FN1) was closely related to S1PR1 due to its colocalization in the endothelial cells of human skin. Further study supported that FN1 plays an important role in the PRP-Exos-S1P-mediated S1PR1/protein kinase B signalling pathway.ConclusionsPRP-Exos-S1P promotes angiogenesis in diabetic wound healing via the S1PR1/protein kinase B/FN1 signalling pathway. Our findings provide a preliminary theoretical foundation for the treatment of diabetic foot ulcers using PRP-Exos in the future.

关键词

Keywords

ExosomesPlatelet-rich plasmaDiabetic foot ulcerWound healingSphingosine-1-phosphate

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