Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration

Ye Li; Xin Bi; Mengfan Wu; Xinyao Chen; Weiqing Zhan; Ziqing Dong; Feng Lu

doi:10.1093/burnst/tkad002

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Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration

更新时间：2023-05-16

- Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration
- Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration
- Burns & Trauma 2023年11卷第1期
- 作者机构：
  
  1. Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University
  2. Dermatology Department, The First People's Hospital of Yunnan Province
  3. Department of Plastic and Cosmetic Surgery, Third Affiliated Hospital of Southern Medical University
- 作者简介：
- 基金信息：
  
  This work was supported by the following organizations: National Nature Science Foundation of China (81601702, 81671931, 81772101, 81701920, 81801933, 81801932, 81871573, 81901976, 81901975, 81971852, 8207081184, 82002052);Natural Science Foundation of Guangdong Province of China (2021A1515011623);Medical Scientific Research Foundation of Guangdong Province of China (A2020542);Science and Technology Program of Guangzhou of China (201604020007);Fundamental and applied fundamental Research Regional United Fund of Guangdong Province (2019A1515110112);Administrator Foundation of Nanfang Hospital (2019B021, 2020Z004);and the National Undergraduate Innovation and Entrepreneurship Training Program (X202012121222, X202012121312).
- DOI：10.1093/burnst/tkad002
  中图分类号：
- 纸质出版日期：2023，
  
  网络出版日期：2023-3-1，
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Ye Li, Xin Bi, Mengfan Wu, 等. Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration[J]. Burns & Trauma, 2023,11(1).

Ye Li, Xin Bi, Mengfan Wu, Xinyao Chen, Weiqing Zhan, Ziqing Dong, Feng Lu, Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration, Burns & Trauma, Volume 11, 2023, tkad002, https://doi.org/10.1093/burnst/tkad002
Ye Li, Xin Bi, Mengfan Wu, 等. Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration[J]. Burns & Trauma, 2023,11(1). DOI： 10.1093/burnst/tkad002.

Ye Li, Xin Bi, Mengfan Wu, Xinyao Chen, Weiqing Zhan, Ziqing Dong, Feng Lu, Adjusting the stiffness of a cell-free hydrogel system based on tissue-specific extracellular matrix to optimize adipose tissue regeneration, Burns & Trauma, Volume 11, 2023, tkad002, https://doi.org/10.1093/burnst/tkad002 DOI：

摘要

Abstract

BackgroundLarge-area soft tissue defects are challenging to reconstruct. Clinical treatment methods are hampered by problems associated with injury to the donor site and the requirement for multiple surgical procedures. Although the advent of decellularized adipose tissue (DAT) offers a new solution to these problems

optimal tissue regeneration efficiency cannot be achieved because the stiffness of DAT cannot be altered in vivo by adjusting its concentration. This study aimed to improve the efficiency of adipose regeneration by physically altering the stiffness of DAT to better repair large-volume soft tissue defects.MethodsIn this study

we formed three different cell-free hydrogel systems by physically cross-linking DAT with different concentrations of methyl cellulose (MC; 0.05

0.075 and 0.10 g/ml). The stiffness of the cell-free hydrogel system could be regulated by altering the concentration of MC

and all three cell-free hydrogel systems were injectable and moldable. Subsequently

the cell-free hydrogel systems were grafted on the backs of nude mice. Histological

immunofluorescence and gene expression analyses of adipogenesis of the grafts were performed on days 3

21 and 30.ResultsThe migration of adipose-derived stem cells (ASCs) and vascularization were higher in the 0.10 g/ml group than in the 0.05 and 0.075 g/ml groups on days 7

14 and 30. Notably

on days 7

14 and 30

the adipogenesis of ASCs and adipose regeneration were significantly higher in the 0.075 g/ml group than in the 0.05 g/ml group (p < 0.01 or p < 0.001) and 0.10 g/ml group (p < 0.05 or p < 0.001).ConclusionAdjusting the stiffness of DAT via physical cross-linking with MC can effectively promote adipose regeneration

which is of great significance to the development of methods for the effective repair and reconstruction of large-volume soft tissue defects.

关键词

Keywords

Extracellular matrixAdipose tissueRegenerationAdipose-derived stem cellsHydrogelStiffness

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