Collagen triple helix repeat containing-1 promotes functional recovery of sweat glands by inducing adjacent microvascular network reconstruction in vivo

Xingyu Yuan; Xianlan Duan; Zhao Li; Bin Yao; Enhejirigala; Wei Song; Yi Kong; Yuzhen Wang; Fanliang Zhang; Liting Liang; Shijun Zhu; Mengde Zhang; Chao Zhang; Sha Huang; Xiaobing Fu

doi:10.1093/burnst/tkac035

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Collagen triple helix repeat containing-1 promotes functional recovery of sweat glands by inducing adjacent microvascular network reconstruction in vivo

更新时间：2023-09-05

- Collagen triple helix repeat containing-1 promotes functional recovery of sweat glands by inducing adjacent microvascular network reconstruction in vivo
- Collagen triple helix repeat containing-1 promotes functional recovery of sweat glands by inducing adjacent microvascular network reconstruction in vivo
- Burns & Trauma 2022年10卷第1期
- 作者机构：
  
  1. School of Medicine
  2. Research Center for Tissue Repair and Regeneration affiliated to the Medical Innovation Research Department, PLA General Hospital
  3. PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury
  4. Chinese PLA General Hospital and PLA Medical College
  5. Academy of Medical Engineering and Translational Medicine, Tianjin University
  6. College of Graduate, Tianjin Medical University
  7. Institute of Basic Medical Research, Inner Mongolia Medical University
  8. Department of Burn and Plastic Surgery, Air Force Hospital of Chinese PLA Central Theater Command
  9. Research Unit of Trauma Care, Tissue Repair and Regeneration, Chinese Academy of Medical Sciences, 2019RU,051
- 作者简介：
- 基金信息：
  
  This study was supported by grants from the National Natural Science Foundation of China (81830064, 81721092, 32000969, 82002056)， Key Support Program for Growth Factor Research (SZYZ-TR-03), Chinese PLA General Hospital for Military Medical Innovation Research Project (CX-19026), the CAMS Innovation Fund for Medical Sciences (CIFMS, 2019-I2M-5-059), the Military Medical Research and Development Projects (AWS17J005) and Beijing Natural Science Foundation (7204306).
- DOI：10.1093/burnst/tkac035
  中图分类号：
- 纸质出版日期：2022，
  
  网络出版日期：2022-8-2，
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Collagen triple helix repeat containing-1 promotes functional recovery of sweat glands by inducing adjacent microvascular network reconstruction in vivo[J]. Burns & Trauma, 2022,10(1).

Xingyu Yuan, Xianlan Duan, Zhao Li, Bin Yao, Enhejirigala, Wei Song, Yi Kong, Yuzhen Wang, Fanliang Zhang, Liting Liang, Shijun Zhu, Mengde Zhang, Chao Zhang, Sha Huang, Xiaobing Fu, Collagen triple helix repeat containing-1 promotes functional recovery of sweat glands by inducing adjacent microvascular network reconstruction in vivo, Burns & Trauma, Volume 10, 2022, tkac035, https://doi.org/10.1093/burnst/tkac035
Collagen triple helix repeat containing-1 promotes functional recovery of sweat glands by inducing adjacent microvascular network reconstruction in vivo[J]. Burns & Trauma, 2022,10(1). DOI： 10.1093/burnst/tkac035.

Xingyu Yuan, Xianlan Duan, Zhao Li, Bin Yao, Enhejirigala, Wei Song, Yi Kong, Yuzhen Wang, Fanliang Zhang, Liting Liang, Shijun Zhu, Mengde Zhang, Chao Zhang, Sha Huang, Xiaobing Fu, Collagen triple helix repeat containing-1 promotes functional recovery of sweat glands by inducing adjacent microvascular network reconstruction in vivo, Burns & Trauma, Volume 10, 2022, tkac035, https://doi.org/10.1093/burnst/tkac035 DOI：

摘要

Abstract

BackgroundSweat glands (SGs) have low regenerative potential after severe burns or trauma and their regeneration or functional recovery still faces many obstacles. In practice

restoring SG function requires not only the structural integrity of the gland itself

but also its neighboring tissues

especially blood vessels. Collagen triple helix repeat containing-1 (CTHRC1) was first identified in vascular repair

and increasing reports showed a close correlation between cutaneous appendage specification

patterning and regeneration. The purpose of the present study was to clarify the role of CTHRC1 in SGs and their adjacent microvessels and find therapeutic strategies to restore SG function.MethodsThe SGs and their adjacent microvascular network of Cthrc1−/− mice were first investigated using sweat test

laser Doppler imaging

tissue clearing technique and transcriptome analysis. The effects of CTHRC1 on dermal microvascular endothelial cells (DMECs) were further explored with cell proliferation

DiI-labeled acetylated low-density lipoprotein uptake

tube formation and intercellular junction establishment assays. The effects of CTHRC1 on SG function restoration were finally confirmed by replenishing the protein into the paws of Cthrc1−/− mice.ResultsCTHRC1 is a key regulator of SG function in mice. At the tissue level

Cthrc1 deletion resulted in the disorder and reduction of the microvascular network around SGs. At the molecular level

the knockout of Cthrc1 reduced the expression of vascular development genes and functional proteins in the dermal tissues. Furthermore

CTHRC1 administration considerably enhanced SG function by inducing adjacent vascular network reconstruction.ConclusionsCTHRC1 promotes the development

morphogenesis and function execution of SGs and their neighboring vasculature. Our study provides a novel target for the restoration or regeneration of SG function in vivo.

关键词

Keywords

Collagen triple helix repeat containing-1Sweat glandsMicrovascular networkDermal microvascular endothelial cells Reconstruction

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Using bioprinting and spheroid culture to create a skin model with sweat glands and hair follicles