P311 promotes type II transforming growth factor-β receptor mediated fibroblast activation and granulation tissue formation in wound healing

Jue Wang; Ruoyu Shang; Jiacai Yang; Zhihui Liu; Yunxia Chen; Cheng Chen; Wenxia Zheng; Yuanyang Tang; Xiaorong Zhang; Xiaohong Hu; Yong Huang; Han-Ming Shen; Gaoxing Luo; Weifeng He

doi:10.1093/burnst/tkac027

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P311 promotes type II transforming growth factor-β receptor mediated fibroblast activation and granulation tissue formation in wound healing

更新时间：2023-09-05

- P311 promotes type II transforming growth factor-β receptor mediated fibroblast activation and granulation tissue formation in wound healing
- P311 promotes type II transforming growth factor-β receptor mediated fibroblast activation and granulation tissue formation in wound healing
- Burns & Trauma 2022年10卷第1期
- 作者机构：
  
  1. State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University)
  2. Chongqing Key Laboratory for Disease Proteomics
  3. Department of Technical Support, Chengdu Zhijing Technology Co., Ltd
  4. Academy of Biological Engineering, Chongqing University
  5. Faculty of Health Sciences, University of Macau
- 作者简介：
- 基金信息：
- DOI：10.1093/burnst/tkac027
  中图分类号：
- 纸质出版日期：2022，
  
  网络出版日期：2022-10-13，
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P311 promotes type II transforming growth factor-β receptor mediated fibroblast activation and granulation tissue formation in wound healing[J]. Burns & Trauma, 2022,10(1).

Jue Wang, Ruoyu Shang, Jiacai Yang, Zhihui Liu, Yunxia Chen, Cheng Chen, Wenxia Zheng, Yuanyang Tang, Xiaorong Zhang, Xiaohong Hu, Yong Huang, Han-Ming Shen, Gaoxing Luo, Weifeng He, P311 promotes type II transforming growth factor-β receptor mediated fibroblast activation and granulation tissue formation in wound healing, Burns & Trauma, Volume 10, 2022, tkac027, https://doi.org/10.1093/burnst/tkac027
P311 promotes type II transforming growth factor-β receptor mediated fibroblast activation and granulation tissue formation in wound healing[J]. Burns & Trauma, 2022,10(1). DOI： 10.1093/burnst/tkac027.

Jue Wang, Ruoyu Shang, Jiacai Yang, Zhihui Liu, Yunxia Chen, Cheng Chen, Wenxia Zheng, Yuanyang Tang, Xiaorong Zhang, Xiaohong Hu, Yong Huang, Han-Ming Shen, Gaoxing Luo, Weifeng He, P311 promotes type II transforming growth factor-β receptor mediated fibroblast activation and granulation tissue formation in wound healing, Burns & Trauma, Volume 10, 2022, tkac027, https://doi.org/10.1093/burnst/tkac027 DOI：

摘要

Abstract

BackgroundP311

a highly conserved 8 kDa intracellular protein

has recently been reported to play an important role in aggravating hypertrophic scaring by promoting the differentiation and secretion of fibroblasts. Nevertheless

how P311 regulates the differentiation and function of fibroblasts to affect granulation tissue formation remains unclear. In this work

we studied the underlying mechanisms via which P311 affects fibroblasts and promotes acute skin wound repair.MethodsTo explore the role of P311

both in vitro and in vivo wound-healing models were used. Full-thickness skin excisional wounds were made in wild-type and P311−/− C57 adult mice. Wound healing rate

re-epithelialization

granulation tissue formation and collagen deposition were measured at days 3

6 and 9 after skin injury. The biological phenotypes of fibroblasts

the expression of target proteins and relevant signaling pathways were examined both in vitro and in vivo.ResultsP311 could promote the proliferation and differentiation of fibroblasts

enhance the ability of myofibroblasts to secrete extracellular matrix and promote cell contraction

and then facilitate the formation of granulation tissue and eventually accelerate skin wound closure. Importantly

we discovered that P311 acts via up-regulating the expression of type II transforming growth factor-β receptor (TGF-βRII) in fibroblasts and promoting the activation of the TGF-βRII-Smad signaling pathway. Mechanistically

the mammalian target of rapamycin signaling pathway is closely implicated in the regulation of the TGF-βRII-Smad pathway in fibroblasts mediated by P311.ConclusionsP311 plays a critical role in activation of the TGF-βRII-Smad pathway to promote fibroblast proliferation and differentiation as well as granulation tissue formation in the process of skin wound repair.

关键词

Keywords

P311Wound healingFibroblastsTGF-βRII-Smad pathwayre-epithelializationGranulation tissue formationCollagen deposition

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